EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development
文献类型：期刊论文
| 作者 | Wang, Bo; Mo, Zhao Lan; Xiao, Peng; Li, Jie ; Zou, Yu Xia; Hao, Bin; Li, Gui Yang
|
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 12期号:6页码:678-685 |
| 关键词 | E. tarda eseD mutant Pathogenesis Recombinant EseD Protective immunity |
| 英文摘要 | Edwardsiella tarda has a type III secretion system (T3SS) essential for pathogenesis. EseD, together with EseB and EseC, has been suggested to form a putative T3SS translocon complex, although its further function is unclear. To investigate the physiological role of EseD, a mutant strain of E. tarda was constructed with an in-frame deletion of the entire eseD gene. One finding was that the a dagger eseD mutant decreased the secretion levels of EseC and EseB proteins. Additionally, the a dagger eseD mutant showed attenuated swarming and contact-hemolysis abilities. However, the a dagger eseD mutant showed increased biofilm formation. Complementation of the mutant strain with eseD restored these phenotypes to those similar to the wild-type strain. Furthermore, infection experiments in fish showed that the a dagger eseD mutant exhibited slower proliferation and a tenfold decrease in virulence in fish. These results indicate a specific role of EseD in the pathogenesis of E. tarda. Finally, recombinant EseD protein elicited high antibody titers in immunized fish and various levels of protection against lethal challenge with the wild-type strain. These results indicate that EseD protein may be a candidate antigen for development of a subunit vaccine against Edwardsiellosis. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284599000006 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32740]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生物分类与系统演化实验室 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Bo,Mo, Zhao Lan,Xiao, Peng,et al. EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development[J]. MARINE BIOTECHNOLOGY,2010,12(6):678-685. |
| APA | Wang, Bo.,Mo, Zhao Lan.,Xiao, Peng.,Li, Jie.,Zou, Yu Xia.,...&Li, Gui Yang.(2010).EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development.MARINE BIOTECHNOLOGY,12(6),678-685. |
| MLA | Wang, Bo,et al."EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development".MARINE BIOTECHNOLOGY 12.6(2010):678-685. |
入库方式： OAI收割
来源：海洋研究所
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