DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda
文献类型：期刊论文
| 作者 | Sun, Kun1,2; Jiao, Xu-dong1,3; Zhang, Min1; Sun, Li1 |
| 刊名 | VETERINARY MICROBIOLOGY
 |
| 出版日期 | 2010-02-24 |
| 卷号 | 141期号:1-2页码:149-154 |
| 关键词 | Antisense RNA DNA adenine methylase Edwardsiella tarda RNA interference Virulence |
| 英文摘要 | Edwardsiella tarda is a serious aquaculture pathogen that can infect many Cultured fish species. The aim of this study was to investigate the potential importance of DNA adenine methylase (Dam) in E. tarda pathogenesis. The E. tardo dam gene (dam(Et)) was cloned from a pathogenic strain, TXD1, isolated from diseased fish. Dam(Et) shares high (70.2%) sequence identity with the Dam proteins of Yersinia enterocolitica and several other bacterial species. Recombinant Dam(Et) is able to complement a dam-deficient Escherichia coli strain and methylate the genomic DNA. Attenuation of dam(Et) expression by antisense RNA interference had no apparent effect on the growth of TXD1, but caused significant attenuation of overall bacterial virulence and altered several stress responses including spontaneous mutation, recovering from UV radiation and H(2)O(2) exposure, binding to host mucus, and dissemination in host blood and liver. In addition, attenuation of dam(Et) expression increased luxS expression and AI-2 activities in E. tarda. These results indicate that Dam(Et) is a virulence determinant and plays a role in the pathogenesis of TXD1, and that temporal expression of dam(Et), is essential for optimal bacterial infection. (C) 2009 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275610200022 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32755]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Polar Res Inst China, Key Lab Polar Sci, State Ocean Adm, Shanghai 200136, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Kun,Jiao, Xu-dong,Zhang, Min,et al. DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda[J]. VETERINARY MICROBIOLOGY,2010,141(1-2):149-154. |
| APA | Sun, Kun,Jiao, Xu-dong,Zhang, Min,&Sun, Li.(2010).DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda.VETERINARY MICROBIOLOGY,141(1-2),149-154. |
| MLA | Sun, Kun,et al."DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda".VETERINARY MICROBIOLOGY 141.1-2(2010):149-154. |
入库方式： OAI收割
来源：海洋研究所
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