Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro
文献类型：期刊论文
| 作者 | Zhao, Xiangzhong1,2; Liu, Ming1; Wu, Ning1; Ding, Lili1; Liu, Haizhou1; Lin, Xiukun1 |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2010-08-01 |
| 卷号 | 72期号:2页码:262-266 |
| 关键词 | p53 Zebrafish RNA binding protein Protein expression Inclusion body |
| 英文摘要 | p53 protein is an important regulation factor that can bind to p53 mRNA to regulate its translation in human and murine. To determine if a similar interaction exists in zebrafish and if the interaction affects zebrafish development, we cloned and expressed p53 protein from zebrafish in Escherichia coli. Soluble p53 protein with high purity was successfully obtained using the optimized renaturation approach. Results of a UV-crosslinking experiment and immunoprecipitation:RT-PCR analysis confirmed that the purified p53 protein could bind specifically to its cognate mRNA. Our results suggest that selecting a suitable buffer is important for renaturing p53 protein from inclusion bodies. We also demonstrated a specific interaction between p53 and it own RNA in zebrafish. Measurement of the binding activity may be a useful approach for identifying the activity of recombinant p53 protein in vitro. (C) 2010 Elsevier Inc. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278193200017 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32772]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xiangzhong,Liu, Ming,Wu, Ning,et al. Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro[J]. PROTEIN EXPRESSION AND PURIFICATION,2010,72(2):262-266. |
| APA | Zhao, Xiangzhong,Liu, Ming,Wu, Ning,Ding, Lili,Liu, Haizhou,&Lin, Xiukun.(2010).Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro.PROTEIN EXPRESSION AND PURIFICATION,72(2),262-266. |
| MLA | Zhao, Xiangzhong,et al."Recovery of recombinant zebrafish p53 protein from inclusion bodies and its binding activity to p53 mRNA in vitro".PROTEIN EXPRESSION AND PURIFICATION 72.2(2010):262-266. |
入库方式： OAI收割
来源：海洋研究所
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