Identification of the S-genotypes of several sweet cherry (Prunus avium L.) cultivars by AS-PCR and pollination
文献类型：期刊论文
| 作者 | Wang, Hongxia1,2; Zhang, KaiChun3; Su, Huairui1; Naihaoye1,4 |
| 刊名 | AFRICAN JOURNAL OF AGRICULTURAL RESEARCH
 |
| 出版日期 | 2010-02-04 |
| 卷号 | 5期号:3页码:250-256 |
| 关键词 | Self-incompatibility Prunus avium S-RNase AS-PCR pollination test |
| 英文摘要 | Sweet cherry cultivars display a self-incompatibility system that restricts self-fertilization and fertilization between cultivars bearing identical S-alleles. PCR-based S-allele typing system for sweet cherry cultivars has been recently developed. It has been reported that all known self-incompatibility ( S) alleles of sweet cherry were more than 16 based on the AS-PCR analysis. In this work, two sets of AS-PCR primers that were designed based on conserved domains of cDNA sequences of S-RNases has been used to characterize the S-genotype of 38 sweet cherry cultivars, including 15 cultivars whose S-genotype had not been previously described. Pollination test was also performed to validate the PCR results, and the consistent results were got. A wide variation in the frequency of S-alleles in the Sweet cherry germplasm was observed. S(3) was the most common in the cultivars evaluated, and S(7), S(10)-S(16), S(23)-S(25) as rare allele was not found in China geographical areas in our study. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275379800011 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32790]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 10093, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hongxia,Zhang, KaiChun,Su, Huairui,et al. Identification of the S-genotypes of several sweet cherry (Prunus avium L.) cultivars by AS-PCR and pollination[J]. AFRICAN JOURNAL OF AGRICULTURAL RESEARCH,2010,5(3):250-256. |
| APA | Wang, Hongxia,Zhang, KaiChun,Su, Huairui,&Naihaoye.(2010).Identification of the S-genotypes of several sweet cherry (Prunus avium L.) cultivars by AS-PCR and pollination.AFRICAN JOURNAL OF AGRICULTURAL RESEARCH,5(3),250-256. |
| MLA | Wang, Hongxia,et al."Identification of the S-genotypes of several sweet cherry (Prunus avium L.) cultivars by AS-PCR and pollination".AFRICAN JOURNAL OF AGRICULTURAL RESEARCH 5.3(2010):250-256. |
入库方式： OAI收割
来源：海洋研究所
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