Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis
文献类型：期刊论文
| 作者 | Mu, Changkao1,2; Zheng, Peilin1; Zhao, Jianmin1; Wang, Lingling1; Zhang, Huan1; Qiu, Limei1; Gai, Yunchao1; Song, Linsheng1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 34期号:7页码:734-740 |
| 关键词 | Eriocheir sinensis Crustin WAP domain Recombinant expression Antibacterial activity Minimal inhibitory concentration (MIC) |
| 英文摘要 | Crustin is a cysteine-rich antibacterial peptide which widely distributes within decapod crustaceans. In the present study, the cDNA encoding crustin-like peptide (designated CrusEs) was cloned from Chinese mitten crab Eriocheir sinensis by using rapid amplification of cDNA ends (RACE) approaches and expressed sequence tag analysis. The full-length cDNA of CrusEs was of 796 bp, containing a 5' untranslated region (UTR) of 214 bp, a 3' UTR of 267 bp with a poly(A) tail, and an open reading frame (ORF) of 315 bp encoding a polypeptide of 104 amino acids including a putative signal peptide of 21 amino acids. A WAP domain and the consensus framework existing in class I crustins were both identified in CrusEs, suggesting that CrusEs is a new member of type I crustins. Quantitative real-time RT-PCR was employed to examine the expression of CrusEs, and its mRNA transcript was mainly detected in haemocytes and gill. The CrusEs mRNA transcript in haemocytes was down-regulated after the challenge with Gram-positive bacteria Micrococcus luteus, while Gram-negative bacteria Listonella anguillarum did not induce significant variation of CrusEs mRNA. The mature peptide of CrusEs was cloned into pET-21a(+) with a C-terminal hexa-histidine tag fused in-frame, and expressed in Escherichia coli, and the recombinant CrusEs inhibited the growth of Gram-positive bacteria with low MIC. The results indicate that CrusEs is a potent antibacterial protein against Gram-positive bacteria infection, and it may play an important role in innate immune response of E. sinensis. (C) 2010 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277891600006 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32793]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Changkao,Zheng, Peilin,Zhao, Jianmin,et al. Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2010,34(7):734-740. |
| APA | Mu, Changkao.,Zheng, Peilin.,Zhao, Jianmin.,Wang, Lingling.,Zhang, Huan.,...&Song, Linsheng.(2010).Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,34(7),734-740. |
| MLA | Mu, Changkao,et al."Molecular characterization and expression of a crustin-like gene from Chinese mitten crab, Eriocheir sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 34.7(2010):734-740. |
入库方式： OAI收割
来源：海洋研究所
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