Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica
文献类型：期刊论文
| 作者 | Liu, Fuli1,2; Wang, Xiuliang1; Yao, Jianting1; Fu, Wandong1,2; Duan, Delin1 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 22期号:1页码:109-111 |
| 关键词 | Phaeophyta Microsatellite EST Molecular marker |
| 英文摘要 | Expressed sequence tag-derived microsatellite markers (EST-SSR) were generated and characterized in Laminaria japonica using data mining from updated public EST databases and polymorphism testing. Fifty-eight of 578 ESTs (10.0%) containing various repeat motifs were used to design polymerase chain reaction (PCR) amplification primers. A total of 12 pairs of primer were generated and used in the PCR amplification. Alleles per locus ranged from two to ten (average of 5.7). The observed heterozygosities and expected heterozygosities were from 0.045 to 0.543 and from 0.056 to 0.814, respectively. All loci were in Hardy-Weinberg equilibrium and no linkage disequilibrium was detected. These robust, informative, and potentially transferable polymorphic markers appear suitable for population, genetic, parentage, and mapping studies of L. japonica. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274435300013 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32794]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100059, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Fuli,Wang, Xiuliang,Yao, Jianting,et al. Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):109-111. |
| APA | Liu, Fuli,Wang, Xiuliang,Yao, Jianting,Fu, Wandong,&Duan, Delin.(2010).Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica.JOURNAL OF APPLIED PHYCOLOGY,22(1),109-111. |
| MLA | Liu, Fuli,et al."Development of expressed sequence tag-derived microsatellite markers for Saccharina (Laminaria) japonica".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):109-111. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。