Molecular characterization and expression analysis of chitinase (Fcchi-3) from Chinese shrimp, Fenneropenaeus chinensis
文献类型：期刊论文
| 作者 | Zhang, Jiquan1; Sun, Yuying2; Li, Fuhua1; Huang, Bingxin1; Xiang, Jianhai1
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| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 37期号:4页码:1913-1921 |
| 关键词 | Fenneropenaeus chinensis Chitinase Recombinant expression LC-ESI-MS |
| 英文摘要 | Complementary DNA (cDNA) and genomic DNA, including flanking regions of the chitinase gene (Fcchi-3) of Fenneropenaeus chinensis, were cloned and sequenced. Fcchi-3 was found to have 92.0 and 91.4% identity at the cDNA level to that of Litopenaeus vannamei and Marsupenaeus japonicus, respectively. The predicted amino acid sequence had an overall similarity with a comparable region of L. vannamei (96.8%) and M. japonicus (93.4%). Based on the cDNA sequence, the genomic structure of the gene was characterized. Sequence analysis revealed that the Fcchi-3 gene was composed of seven exons with 411, 252, 186, 132, 171, 117 and 135 bp and six introns with 232, 196, 121, 90, 159 and 157 bp. Analysis by RT-PCR revealed that Fcchi-3 was a hepatopancreas specific gene. Semi-quantitative RT-PCR analysis revealed that Fcchi-3 transcript was down-regulated significantly in response to the challenge of WSSV at 5 h post-injection and then came back to normal level at 37 h. A fusion protein containing Fcchi-3 was produced and the purified recombinant protein exhibited similar biological function. The result of identification through LC-ESI-MS showed that three peptide fragments (-MAADPVLR-, -ATIDPAYNVPELSK- and -AILAVGGWNEGSPK-) of the recombinant protein were identical to the corresponding sequence of L. vannamei chitinase-3. The recombinant Fcchi-3 could degrade the colloid chitin confirming that the recombinant protein is actually the chitinase. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275161700029 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32810]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jiquan,Sun, Yuying,Li, Fuhua,et al. Molecular characterization and expression analysis of chitinase (Fcchi-3) from Chinese shrimp, Fenneropenaeus chinensis[J]. MOLECULAR BIOLOGY REPORTS,2010,37(4):1913-1921. |
| APA | Zhang, Jiquan,Sun, Yuying,Li, Fuhua,Huang, Bingxin,&Xiang, Jianhai.(2010).Molecular characterization and expression analysis of chitinase (Fcchi-3) from Chinese shrimp, Fenneropenaeus chinensis.MOLECULAR BIOLOGY REPORTS,37(4),1913-1921. |
| MLA | Zhang, Jiquan,et al."Molecular characterization and expression analysis of chitinase (Fcchi-3) from Chinese shrimp, Fenneropenaeus chinensis".MOLECULAR BIOLOGY REPORTS 37.4(2010):1913-1921. |
入库方式： OAI收割
来源：海洋研究所
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