An immune responsive multidomain galectin from bay scallop Argopectens irradians
文献类型：期刊论文
| 作者 | Song, Xiaoyan1,2; Zhang, Huan1; Zhao, Jianmin1; Wang, Lingling1; Qiu, Limei1; Mu, Changkao1; Liu, Xiaolin2; Qiu, Lihua3; Song, Linsheng1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 28期号:2页码:326-332 |
| 关键词 | Argopectens irradians Galectin mRNA expression Quantitative real-time PCR Bacteria and yeast challenge |
| 英文摘要 | Galectins are a family of P-galactoside-binding lectins which play crucial roles in innate immunity of vertebrates and invertebrates. In the present study, the cDNA of a galectin with multiple carbohydrate-recognition domains (CRDs) was cloned from bay scallop Argopectens irradians (designated AiGal1) by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of AiGal1 was of 2235 nucleotides, encoding a polypeptide of 549 amino acids. SMART program analysis revealed that AiGal1 contained four galectin CRDs, and all the CRDs contained the two consensus motifs essential for ligand-binding. Quantitative real-time PCR was employed to investigate the tissue distribution of AiGal1 mRNA and temporal expression in haemocytes of scallops challenged with Vibrio anguillarum, Micrococcus luteus and Pichia pastoris. The AiGal1 mRNA could be detected in all tested tissues with the highest expression level in hepatopancreas. After challenged by V. anguillarum and M. luteus, the expression level of AiGal1 mRNA was both up-regulated and reached the maximum level at 9 h (1.52 fold, P < 0.05) and 18 h (2.89 fold, P < 0.01) post challenge, respectively. However, there was no significant difference in the mRNA expression of AiGal1 in haemocytes after P. pastoris challenge (P > 0.05). These results collectively indicated that AiGal1 was a new member of the galectin family and involved in the immune responses against bacterial infection. (C) 2009 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274721300011 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32813]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China 3.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Xiaoyan,Zhang, Huan,Zhao, Jianmin,et al. An immune responsive multidomain galectin from bay scallop Argopectens irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(2):326-332. |
| APA | Song, Xiaoyan.,Zhang, Huan.,Zhao, Jianmin.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2010).An immune responsive multidomain galectin from bay scallop Argopectens irradians.FISH & SHELLFISH IMMUNOLOGY,28(2),326-332. |
| MLA | Song, Xiaoyan,et al."An immune responsive multidomain galectin from bay scallop Argopectens irradians".FISH & SHELLFISH IMMUNOLOGY 28.2(2010):326-332. |
入库方式： OAI收割
来源：海洋研究所
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