Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification
文献类型：期刊论文
| 作者 | Gao Hongwei1,2; Li Fuhua1; Zhang Xiaojun1; Wang Bing1; Xiang Jianhai1
|
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2010 |
| 卷号 | 28期号:1页码:62-66 |
| 关键词 | Loop-Mediated Isothermal Amplification (LAMP) detection assay empA gene Vibrio anguillarum |
| 英文摘要 | Vibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65 degrees C in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V. anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275654100008 |
| 公开日期 | 2015-07-28 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32827]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Shandong Entry Exit Inspect & Quarantine Bur Peop, Qingdao 266001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao Hongwei,Li Fuhua,Zhang Xiaojun,et al. Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(1):62-66. |
| APA | Gao Hongwei,Li Fuhua,Zhang Xiaojun,Wang Bing,&Xiang Jianhai.(2010).Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(1),62-66. |
| MLA | Gao Hongwei,et al."Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.1(2010):62-66. |
入库方式： OAI收割
来源：海洋研究所
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