Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis
文献类型：期刊论文
| 作者 | Su, Hai-Nan1; Xie, Bin-Bin1; Chen, Xiu-Lan1; Wang, Jin-Xia2; Zhang, Xi-Ying1; Zhou, Bai-Cheng1,2; Zhang, Yu-Zhong1 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 22期号:1页码:65-70 |
| 关键词 | Allophycocyanin Purification Hydroxylapatite Anion-exchange chromatography Spirulina platensis |
| 英文摘要 | Allophycocyanin (APC) is a minor component of phycobiliproteins in cyanobacteria and red algae. This paper describes a simple and inexpensive extracting method for isolating APC from Spirulina (Arthrospira) platensis with high efficiency. The crude phycobiliprotein extract was pretreated by ammonium sulfate fractionation. Then, by adding hydroxylapatite into crude phycobiliprotein extract dissolved in 20 mM phosphate buffer (pH 7.0), APC was selectively adsorbed by hydroxylapatite but C-phycocyanin (C-PC) was not. The hydroxylapatite was collected and APC was extracted from the crude phycobiliprotein extract. Then, the enriched APC was washed off from the hydroxylapatite using 100 mM phosphate buffer (pH 7.0). In this simple extracting method it was easy to remove C-PC and isolate APC in large amounts. The absorbance ratio A (650)/A (280) of extracted APC reached 2.0. The recovery yield was 70%, representing 4.61 mg center dot g(-1) wet weight. The extracted APC could be further purified by a simple anion-exchange chromatography with a pH gradient from 5.6 to 4.0. The absorbance ratio A (650)/A (280) of the purified APC reached 5.0, and the overall recovery yield was 43%, representing 2.83 mg center dot g(-1) wet weight. Its purity was confirmed by native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-PAGE. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274435300007 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32838]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Shandong Univ, State Key Lab Microbial Technol, Marine Biotechnol Res Ctr, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Hai-Nan,Xie, Bin-Bin,Chen, Xiu-Lan,et al. Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):65-70. |
| APA | Su, Hai-Nan.,Xie, Bin-Bin.,Chen, Xiu-Lan.,Wang, Jin-Xia.,Zhang, Xi-Ying.,...&Zhang, Yu-Zhong.(2010).Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis.JOURNAL OF APPLIED PHYCOLOGY,22(1),65-70. |
| MLA | Su, Hai-Nan,et al."Efficient separation and purification of allophycocyanin from Spirulina (Arthrospira) platensis".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):65-70. |
入库方式： OAI收割
来源：海洋研究所
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