Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Yao, Cui-Luan2; Ji, Pei-Feng2; Wang, Zhi-Yong2; Li, Fu-hua1; Xiang, Jian-Hai1
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2010-03-01 |
| 卷号 | 28期号:3页码:453-460 |
| 关键词 | NOS Litopenaeus vannamei Expression LPS Poly I C |
| 英文摘要 | The importance of the nitric oxide synthase (NOS) gene family is demonstrated by many studies in recent years However, the lack of sequence information and clones of shrimp NOS cDNA limits further study on its characterization and function in this species In this report, the cDNA of NOS contained full-length ORF was cloned from the Pacific white shrimp, Litopenaeus vannamei It was of 4680 bp, including a 5'-terminal untranslated region (UTR) of 278 bp, a 3'-terminal UTR of 862 bp, which contained 5 ATTTA repeats, and an open reading frame (ORF) of 3540 bp encoding a polypeptide of 1179 amino acids It contained a typical NO synthase domain at the N-terminal, next to a flavodoxin I domain, a flavin adenine dinucleotide (FAD) binding domain, respectively, and a conservative nicotinamide adenine dinucleotide (NAD) binding domain structure at the C-terminal Quantitative real-time reverse transcription PCR analysis revealed L vannamei NOS (LvNOS) to be expressed in most shrimp tissues, with highest expression in the hepatopancreas and weakest expression in skin. The expression of LvNOS after challenge with LPS and poly I:C was tested in hemocytes, hepatopancreas and nerve The results indicated that the NOS transcript level could be induced in hemocytes by injection with LPS The highest expression was in the hemocyte. with 8 8 times (at 3 h) as much as that in the control (p < 0.05) However, sharp clown-regulation of NOS was found in hepatopancreas and nerve after LPS and poly I-C injection (p < 0 05). These results Suggested that NOS might play an important role in shrimp's defense against pathogenic infection. (C) 2009 Elsevier Ltd All rights reserved |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275704900007 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32842]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Jimei Univ, Fisheries Biotechnol Inst, Coll Fisheries, Fujian Prov Univ,Key Lab Sci & Technol Aquacultur, Xiamen 361021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Cui-Luan,Ji, Pei-Feng,Wang, Zhi-Yong,et al. Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(3):453-460. |
| APA | Yao, Cui-Luan,Ji, Pei-Feng,Wang, Zhi-Yong,Li, Fu-hua,&Xiang, Jian-Hai.(2010).Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,28(3),453-460. |
| MLA | Yao, Cui-Luan,et al."Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 28.3(2010):453-460. |
入库方式： OAI收割
来源：海洋研究所
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