Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption
文献类型：期刊论文
| 作者 | Niu, Jian-Feng1; Chen, Zhang-Fan1; Wang, Guang-Ce1,2; Zhou, Bai-Cheng1 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2010-02-01 |
| 卷号 | 22期号:1页码:25-31 |
| 关键词 | Porphyra yezoensis R-phycoerythrin Hydrophobic interaction chromatography Expanded bed absorption Ion-exchange chromatography Streamline column |
| 英文摘要 | R-phycoerythrin was purified by means of phenyl-sepharose expanded bed absorption and DEAE-sepharose ion-exchange chromatography from Porphyra yezoensis, one of the largest and important aquaculture species in China. Final R-phycoerythrin preparation was characterized by purity ratio above 4 and the homogeneity in native PAGE, respectively. The results of absorption spectrum, fluorescence spectrum and SDS-PAGE were in agreement with previous reports on R-PE. The yield of R-phycoerythrin was 0.82 mg g(-1) wet leafy gametophyte of P. yezoensis. This method is a high-protein recovery technology while reducing processing time, and is suitable for the large batch production of R-phycoerythrin, which will enhance the value of P. yezoensis in China, especially the inferior P. yezoensis which can not be used for flake processing. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274435300003 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32844]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Jian-Feng,Chen, Zhang-Fan,Wang, Guang-Ce,et al. Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):25-31. |
| APA | Niu, Jian-Feng,Chen, Zhang-Fan,Wang, Guang-Ce,&Zhou, Bai-Cheng.(2010).Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption.JOURNAL OF APPLIED PHYCOLOGY,22(1),25-31. |
| MLA | Niu, Jian-Feng,et al."Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):25-31. |
入库方式： OAI收割
来源：海洋研究所
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