An inhibitor kappa B homologue from bay scallop Argopecten irradians
文献类型：期刊论文
| 作者 | Mu, Changkao1,2; Yu, Yundong1; Zhao, Jianmin1; Wang, Lingling1; Song, Xiaoyan3; Zhang, Huan1; Qiu, Limei1; Song, Linsheng1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 28期号:4页码:687-694 |
| 关键词 | Argopecten irradians Innate immunity Signaling pathway NF-kappa B I kappa B Gene cloning |
| 英文摘要 | I kappa B is an important member of NF-kappa B pathway in the innate immune system. In the present study, the full-length cDNA sequence encoding I kappa B protein (designated AiI kappa B) was isolated from bay scallop Argopecten irradians. The complete sequence of AiI kappa B cDNA containing a 5' untranslated region (UTR) of 237 bp, a 3' UTR of 1023 bp with a poly (A) tail, and an open reading frame (ORF) of 1086 bp encoding a polypeptide of 361 amino acids with the predicted molecular weight of 39.9 kDa and theoretical isoelectric point of 4.7. Six ankyrin repeats which were necessary for specific binding to NF-kappa B and two potential phosphorylation sites responsible for I kappa B degradation were identified in the N-terminus of AiI kappa B. No PEST domain but a phosphorylation site motif (S(357)DSD(360)) was present at the C-terminus of AiI kappa B. Predicted three-dimensional structure of AiI kappa B shared high similarity with mammalian I kappa B alpha. Similarity and phylogenetic analysis revealed that AiI kappa B was clustered into I kappa Bs from invertebrate. All these typical characteristics indicated that the AiI kappa B should be classified into I kappa B family proteins. Quantitative real-time RT-PCR was employed to assess the mRNA expression of AiI kappa B in various tissues and its temporal expression in haemocytes of scallops challenged with Listonella anguillarum. The mRNA transcript of AiI kappa B could be detected in all the examined tissues with highest expression level in hepatopancreas. Bacteria infection inhibited the transcription level of AiI kappa B. The results suggested the involvement of AiI kappa B in responses against bacterial infection and further highlighted its functional importance in the immune system of A. irradians. (C) 2010 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276935700023 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32851]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China 3.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mu, Changkao,Yu, Yundong,Zhao, Jianmin,et al. An inhibitor kappa B homologue from bay scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(4):687-694. |
| APA | Mu, Changkao.,Yu, Yundong.,Zhao, Jianmin.,Wang, Lingling.,Song, Xiaoyan.,...&Song, Linsheng.(2010).An inhibitor kappa B homologue from bay scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,28(4),687-694. |
| MLA | Mu, Changkao,et al."An inhibitor kappa B homologue from bay scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 28.4(2010):687-694. |
入库方式： OAI收割
来源：海洋研究所
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