Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization
文献类型：期刊论文
| 作者 | Zhang Baoyu1; Chen Guofu2; Wang Guangce1,3; Lu Douding4 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 28期号:2页码:310-314 |
| 关键词 | fluorescent in situ hybridization ITS sequence Skeletonema costatum harmful algal bloom |
| 英文摘要 | A harmful algae bloom (HAB) is a dense aggregation of algae in a marine or aquatic environment that can result in significant environmental problems. To forecast the occurrence of HAB, development of a rapid and precise detection method is urgently required. In this study, two Skeletonema costatum-like diatoms (SK-1 and SK-2), were identified morphologically under a light microscope, and detected using fluorescent in situ hybridization (FISH). Strain SK-1 was isolated from a frequently HAB affected area of the East China Sea, and strain SK-2 from an aquatic farm in Qingdao, China. Fluorescent DNA probes were designed that were complementary to the ITS sequence (including 5.8S rDNA) of strain SK-1. After hybridization, strong green fluorescence was observed in cells of strain SK-1 under an epifluorescence microscope; however, no such fluorescence was observed with strain SK-2, which indicates that probes hybridized only the DNA of the target strain, SK-1, in species-specific manner, and that the two strains do not belong to a same species. This finding was confirmed by ITS sequence analysis. The FISH technique used in this study was sensitive, simple, and rapid, and is a promising tool for detecting target HAB species in natural environments. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275781400016 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32860]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Harbin Inst Technol, Weihai 264209, Peoples R China 3.Tianjin Univ Sci & Technol, Tianjin 300457, Peoples R China 4.SOA, Inst Oceanog 2, Hangzhou 310012, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Baoyu,Chen Guofu,Wang Guangce,et al. Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(2):310-314. |
| APA | Zhang Baoyu,Chen Guofu,Wang Guangce,&Lu Douding.(2010).Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(2),310-314. |
| MLA | Zhang Baoyu,et al."Identification of two Skeletonema costatum-like diatoms by fluorescence in situ hybridization".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.2(2010):310-314. |
入库方式： OAI收割
来源：海洋研究所
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