Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen
文献类型：期刊论文
| 作者 | Jiao, Xu-dong1,2; Zhang, Min1; Cheng, Shuang1,2; Sun, Li1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2010-04-01 |
| 卷号 | 28期号:4页码:672-677 |
| 关键词 | DegP Edwardsiella tarda Immunogen LuxS Protease Virulence |
| 英文摘要 | Edwardsiella tarda is a severe aquaculture pathogen with a broad host range that includes humans, animal, and fish. A gene (degP(Et)) encoding a DegP homologue was cloned from TX01, a pathogenic E. tarda strain isolated from diseased fish. DegP(Et) shares high sequence identities with the DegP proteins of several bacterial species. Functional analyses showed that degP(Et) could complement the temperature-sensitive phenotype of an Escherichia coli degP null mutant. Expression of degP(Et) in TX01 was modulated by growth phase and temperature, the latter possibly through the action of the sigma(E)-like factor. Overexpression of degP(Et) (i) enhanced the ability of TX01 to disseminate in fish blood at the advanced stage of infection. (ii) heightened the activity of type 2 autoinducer, and (iii) increased the expression of luxS and the genes encoding components of the virulence-associated type III secretion system. Recombinant DegP(Et) purified from E. coli was a serine protease that exhibited maximum activity at 40 degrees C and pH8.0. The proteolytic activity of recombinant DegP(Et) depended on the catalytic triad and the PDZ domains. Immunoprotective analyses showed that purified recombinant DegP(Et) was a protective immunogen that could induce the production of specific serum antibodies and elicit strong protective immunity in fish vaccinated with DegP(Et). (C) 2010 Elsevier Ltd. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276935700021 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32894]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiao, Xu-dong,Zhang, Min,Cheng, Shuang,et al. Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(4):672-677. |
| APA | Jiao, Xu-dong,Zhang, Min,Cheng, Shuang,&Sun, Li.(2010).Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen.FISH & SHELLFISH IMMUNOLOGY,28(4),672-677. |
| MLA | Jiao, Xu-dong,et al."Analysis of Edwardsiella tarda DegP, a serine protease and a protective immunogen".FISH & SHELLFISH IMMUNOLOGY 28.4(2010):672-677. |
入库方式： OAI收割
来源：海洋研究所
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