Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers
文献类型：期刊论文
| 作者 | Li, Sanlei1; Xu, Dongdong1; Lou, Bao1; Xu, Hanxiang1; You, Feng2; Zhan, Wei1; Geng, Zhi1 |
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2011-10-05 |
| 卷号 | 10期号:60页码:12833-12839 |
| 关键词 | Japanese flounder amplified fragment length polymorphism (AFLP) cytochrome c oxidase subunit I (COI) gene genetic diversity population structure |
| 英文摘要 | The population structure of Japanese flounder (Paralichthys olivaceus) in the Yellow and East China Seas were analyzed using amplified fragment length polymorphism (AFLP) and cytochrome c oxidase subunit I (COI) gene sequencing. A total of 390 reproducible bands were generated by 10 AFLP primer combinations in two populations collected from the coasts of Qingdao (located at the Yellow Sea) and Zhoushan (located at the East China Sea). The percentage of polymorphic loci (P), Nei's genetic diversity (H) and Shannon's information index (I) values were higher in the Qingdao population (P = 72.85%, H = 0.243 and I = 0.364) than those in the Zhoushan population (P = 56.35%, H = 0.189 and I = 0.284). The genetic diversity reduction in the Zhoushan population may be attributed to fishing pressure and habitat loss in this area. Based on the COI sequencing analysis, a total of 25 polymorphic sites were examined, and 15 haplotypes were identified in the two populations. The haplotype diversity (h) and nucleotide diversity (pi) values in the Qingdao population were 0.746 +/- 0.0728 and 0.00334 +/- 0.00103, respectively. The corresponding values in the Zhoushan population were 0.712 +/- 0.0470 and 0.00318 +/- 0.00049. Both the AFLP and mtDNA data revealed significant genetic differentiation between the two populations. The present study discussed the factors that may result in genetic differentiation between the populations in the Yellow and East China Seas. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298544000006 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/32944]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Marine Fishery Inst Zhejiang Prov, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan 316100, Peoples R China 2.Zhejiang Ocean Univ, Coll Marine Sci, Zhoushan 316100, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Sanlei,Xu, Dongdong,Lou, Bao,et al. Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(60):12833-12839. |
| APA | Li, Sanlei.,Xu, Dongdong.,Lou, Bao.,Xu, Hanxiang.,You, Feng.,...&Geng, Zhi.(2011).Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(60),12833-12839. |
| MLA | Li, Sanlei,et al."Genetic differentiation in Japanese flounder in the Yellow Sea and East China Sea by amplified fragment length polymorphism (AFLP) and mitochondrial DNA markers".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.60(2011):12833-12839. |
入库方式： OAI收割
来源：海洋研究所
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