Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization
文献类型:期刊论文
作者 | Wang, YP; Guo, XM |
刊名 | JOURNAL OF SHELLFISH RESEARCH
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出版日期 | 2001-12-01 |
卷号 | 20期号:3页码:1187-1190 |
关键词 | FISH chromosome telomeric sequence mapping evolution mollusca |
英文摘要 | The presence and chromosomal location of the vertebrate telomeric sequence (TTAGGG)n was studied in four bivalve molluscs: the eastern oyster, Crassostrea virginica Gmelin; the mangrove oyster, Crassostrea rhizophorae Guilding; the hard clam, Mercenaria mercenaria Linnaeus: and the dwarf surfclam, Mulinia lateralis Say, using fluorescence in situ hybridization (FISH). Metaphase chromosomes were prepared from embryos, and the vertebrate telomeric probe was labeled with digoxigenin and detected with fluorescein labeled anti-digoxigenin antibodies. Positive FISH signals were detected in all four species. Signals were restricted to telomeric regions of all chromosomes. and no interstitial sites were observed. Results of this and previous studies suggest that the vertebrate sequence is likely the telomeric sequence for all bivalve molluscs, which so far are the only invertebrates showing the vertebrate lineage in telomeric DNA. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000174127300035 |
源URL | [http://ir.qdio.ac.cn/handle/337002/32987] ![]() |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, YP,Guo, XM. Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization[J]. JOURNAL OF SHELLFISH RESEARCH,2001,20(3):1187-1190. |
APA | Wang, YP,&Guo, XM.(2001).Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization.JOURNAL OF SHELLFISH RESEARCH,20(3),1187-1190. |
MLA | Wang, YP,et al."Chromosomal mapping of the vertebrate telomeric sequence (TTAGGG)N in four bivalve molluscs by fluorescence in situ hybridization".JOURNAL OF SHELLFISH RESEARCH 20.3(2001):1187-1190. |
入库方式: OAI收割
来源:海洋研究所
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