CfLec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate C-type lectin
文献类型：期刊论文
| 作者 | Yang, Jialong1,3; Huang, Mengmeng1,3 ; Zhang, Huan1; Wang, Lingling1; Wang, Hao1; Wang, Leilei1,3; Qiu, Limei1; Song, Linsheng1,2
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2015-05-15 |
| 卷号 | 5 |
| 英文摘要 | A C-type lectin (CfLec-3) from Chlamys farreri with three carbohydrate-recognition domains (CRDs) was selected to dissect the possible mechanisms of PAMP binding and functional differentiation of invertebrate lectins. CfLec-3 distributed broadly, and its mRNA expression in hemocytes increased significantly after stimulations with LPS, PGN or beta-glucan, but not poly(I: C). The recombinant CfLec-3 (rCfLec-3) could bind PAMPs and several microbes. rCfLec-3 mediated hemocytes phagocytosis against Escherichia coli and encapsulation towards agarose beads. Obvious functional differentiation occurred among the three CRDs, as CRD1 exhibited higher activity to bind PAMPs, while CRD2/3 were expert in promoting hemocyte mediated opsonisation. The tertiary structural differences were suspected to be associated with such functional differentiation. PAMP binding abilities of CfLec-3 were determined by Ca2+-binding site 2 motif. When Pro in this motif of each CRD was mutated into Ser, their PAMP binding abilities were deprived absolutely. rCRD2 acquired mannan binding capability when its EPD was replaced by EPN, but lost when EPN in rCRD3 was changed into EPD. The Pro in Ca2+-binding site 2 was indispensable for PAMPs binding, while Asn was determinant for specific binding to mannan. It shed new insight into PAMPs binding mechanism of invertebrate C-type lectins and their functional differentiation. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355247500002 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/33045]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jialong,Huang, Mengmeng,Zhang, Huan,et al. CfLec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate C-type lectin[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Yang, Jialong.,Huang, Mengmeng.,Zhang, Huan.,Wang, Lingling.,Wang, Hao.,...&Song, Linsheng.(2015).CfLec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate C-type lectin.SCIENTIFIC REPORTS,5. |
| MLA | Yang, Jialong,et al."CfLec-3 from scallop: an entrance to non-self recognition mechanism of invertebrate C-type lectin".SCIENTIFIC REPORTS 5(2015). |
入库方式： OAI收割
来源：海洋研究所
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