Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome
文献类型：期刊论文
| 作者 | Zhang, Hao1,2; Li, Fuchao2; Chen, Huaxin2; Zhao, Jin2; Yan, Jinfei3; Jiang, Peng2; Li, Ronggui1; Zhu, Baoli4 |
| 刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 33期号:4页码:819-827 |
| 关键词 | marine sediment metagenome lipolytic expression esterase characterization |
| 英文摘要 | Lipolytic enzymes, including esterases and lipases, represent a group of hydrolases that catalyze the cleavage and formation of ester bonds. A novel esterase gene, scsEst01, was cloned from a South China Sea sediment metagenome. The scsEst01 gene consisted of 921 bp encoding 307 amino acid residues. The predicted amino acid sequence shared less than 90% identity with other lipolytic enzymes in the NCBI nonredundant protein database. ScsEst01 was successfully co-expressed in Escherichia coli BL21 (DE3) with chaperones (dnaK-dnaJ-grpE) to prevent the formation of inclusion bodies. The recombinant protein was purified on an immobilized metal ion affinity column containing chelating Sepharose charged with Ni2+. The enzyme was characterized using p -nitrophenol butyrate as a substrate. ScsEst01 had the highest lipolytic activity at 35A degrees C and pH 8.0, indicative of a meso-thermophilic alkaline esterase. ScsEst01 was thermostable at 20A degrees C. The lipolytic activity of scsEst01 was strongly increased by Fe2+, Mn2+ and 1% Tween 80 or Tween 20. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357037100001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/33091]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Qingdao Univ, Microbiol Lab, Qingdao 266071, Shandong Provin, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.State Ocean Adm, Inst Oceanog 3, Xiamen 361005, Peoples R China 4.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hao,Li, Fuchao,Chen, Huaxin,et al. Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2015,33(4):819-827. |
| APA | Zhang, Hao.,Li, Fuchao.,Chen, Huaxin.,Zhao, Jin.,Yan, Jinfei.,...&Zhu, Baoli.(2015).Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,33(4),819-827. |
| MLA | Zhang, Hao,et al."Cloning, expression and characterization of a novel esterase from a South China Sea sediment metagenome".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 33.4(2015):819-827. |
入库方式： OAI收割
来源：海洋研究所
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