ZnO nanoparticles induced cytotoxicity on human pulmonary adenocarcinoma cell line LTEP-a-2
文献类型：期刊论文
| 作者 | Wang, Caixia; Wang, Hui ; Lin, Meng; Hu, Xiaoke
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| 刊名 | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
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| 出版日期 | 2015 |
| 卷号 | 93页码:265-273 |
| 关键词 | Human pulmonary adenocarcinoma cells ZnO nanoparticles Cytotoxicity Reactive oxygen species Apoptosis |
| ISSN号 | 0957-5820 |
| 产权排序 | [Wang, Caixia; Wang, Hui; Lin, Meng; Hu, Xiaoke] Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Hu, XK (reprint author), 17 Chunhui Rd, Yantai 264003, Peoples R China. xkhu@yic.ac.cn |
| 中文摘要 | Novel nanoparticles (NPs) such as zinc oxide (ZnO) NPs are widely produced and applied in our daily lives at a rapid pace. Thus, the toxicity of ZnO NPs should be monitored as an important standard for environmental risk assessment. Here we assessed the in vitro cytotoxicity of ZnO NPs on human pulmonary adenocarcinoma cells LTEP-a-2 by tetrazolium salt colorimetric assay of cell proliferation in the presence or absence of ZnO NPs. ZnO NPs-induced morphological changes in LTEP-a-2 cells were examined by light and scanning electron microscopy. The mechanism by which ZnO NPs impose the cytotoxic effect was investigated by a combination of active oxygen test, lactose dehydrogenase-release assay, and apoptosis detection. Results showed that ZnO NPs significantly inhibited the proliferation and induced evident morphological changes (cell shrinkage and chromosome condensation) in LTEP-a-2 cells. Additionally, ZnO NPs increased the level of intracellular reactive oxygen species and induced the formation of apoptotic vesicles as well as the lysis of cell nuclei. Zn2+ ions released from ZnO NPs into aqueous solution are important components that exert cytotoxic effects on LTEP-a-2 cells. This study provides new insights to the cytotoxicity of ZnO NPs against human health. (C) 2014 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved. |
| 英文摘要 | Novel nanoparticles (NPs) such as zinc oxide (ZnO) NPs are widely produced and applied in our daily lives at a rapid pace. Thus, the toxicity of ZnO NPs should be monitored as an important standard for environmental risk assessment. Here we assessed the in vitro cytotoxicity of ZnO NPs on human pulmonary adenocarcinoma cells LTEP-a-2 by tetrazolium salt colorimetric assay of cell proliferation in the presence or absence of ZnO NPs. ZnO NPs-induced morphological changes in LTEP-a-2 cells were examined by light and scanning electron microscopy. The mechanism by which ZnO NPs impose the cytotoxic effect was investigated by a combination of active oxygen test, lactose dehydrogenase-release assay, and apoptosis detection. Results showed that ZnO NPs significantly inhibited the proliferation and induced evident morphological changes (cell shrinkage and chromosome condensation) in LTEP-a-2 cells. Additionally, ZnO NPs increased the level of intracellular reactive oxygen species and induced the formation of apoptotic vesicles as well as the lysis of cell nuclei. Zn2+ ions released from ZnO NPs into aqueous solution are important components that exert cytotoxic effects on LTEP-a-2 cells. This study provides new insights to the cytotoxicity of ZnO NPs against human health. (C) 2014 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Engineering, Environmental; Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | CERIUM OXIDE NANOPARTICLES ; OXIDATIVE STRESS ; ZINC-OXIDE ; TITANIUM-DIOXIDE ; IN-VITRO ; PARTICLE-SIZE ; TOXICITY ; GENOTOXICITY ; APOPTOSIS ; NANOMATERIALS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000349274100026 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8518]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Caixia,Wang, Hui,Lin, Meng,et al. ZnO nanoparticles induced cytotoxicity on human pulmonary adenocarcinoma cell line LTEP-a-2[J]. PROCESS SAFETY AND ENVIRONMENTAL PROTECTION,2015,93:265-273. |
| APA | Wang, Caixia,Wang, Hui,Lin, Meng,&Hu, Xiaoke.(2015).ZnO nanoparticles induced cytotoxicity on human pulmonary adenocarcinoma cell line LTEP-a-2.PROCESS SAFETY AND ENVIRONMENTAL PROTECTION,93,265-273. |
| MLA | Wang, Caixia,et al."ZnO nanoparticles induced cytotoxicity on human pulmonary adenocarcinoma cell line LTEP-a-2".PROCESS SAFETY AND ENVIRONMENTAL PROTECTION 93(2015):265-273. |
入库方式： OAI收割
来源：烟台海岸带研究所
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