Optimizing medium for producing ethanol from industrial crop Jerusalem artichoke by one- step fermentation and recombinant Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Du, Y. C.1,2; Long, X. H.1; Liu, Z. P.1; Shao, H. B.1,2,3 |
| 刊名 | PLANT BIOSYSTEMS
 |
| 出版日期 | 2014-01-02 |
| 卷号 | 148期号:1页码:118-126 |
| 关键词 | RSM optimization Jerusalem artichoke energy Saccharomyces cerevisiae one-step fermentation |
| ISSN号 | 1126-3504 |
| 产权排序 | [Du, Y. C.; Long, X. H.; Liu, Z. P.; Shao, H. B.] Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Du, Y. C.; Shao, H. B.] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Shao, H. B.] Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao 266042, Peoples R China |
| 通讯作者 | Long, XH (reprint author), Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China. longxiaohua@njau.edu.cn ; zhaopuliu@163.com ; shaohongbochu@126.com |
| 中文摘要 | In order to obtain a high ethanol yield from the Jerusalem artichoke raw extract and reduce the fermentation cost, we have engineered a new recombinant Saccharomyces cerevisiae strain that could produce ex-inulinase. The response surface methodology based on Plackett-Burman and Box-Behnken design was used to optimize the medium for the ethanol production from the Jerusalem artichoke raw extracts by the recombinant strain. In the first optimization step, Plackett-Burman design was employed to select significant factors, including concentrations of yeast extract, inoculum, and MgSO(4)7H(2)O. In the second step, the steepest ascent experiment was carried out to determine the center point with the three significant factors; the selected combinations were further optimized using the Box-Behnken design. The maximum ethanol production rate was predicted at 91.1g/l, which was based on a medium consisting of yeast extract 9.24g/l, inoculum 39.8ml/l, and MgSO(4)7H(2)O 0.45g/l. In the validating experiment, the ethanol fermentation rate reached 102.1g/l, closely matching the predicted rate. |
| 英文摘要 | In order to obtain a high ethanol yield from the Jerusalem artichoke raw extract and reduce the fermentation cost, we have engineered a new recombinant Saccharomyces cerevisiae strain that could produce ex-inulinase. The response surface methodology based on Plackett-Burman and Box-Behnken design was used to optimize the medium for the ethanol production from the Jerusalem artichoke raw extracts by the recombinant strain. In the first optimization step, Plackett-Burman design was employed to select significant factors, including concentrations of yeast extract, inoculum, and MgSO(4)7H(2)O. In the second step, the steepest ascent experiment was carried out to determine the center point with the three significant factors; the selected combinations were further optimized using the Box-Behnken design. The maximum ethanol production rate was predicted at 91.1g/l, which was based on a medium consisting of yeast extract 9.24g/l, inoculum 39.8ml/l, and MgSO(4)7H(2)O 0.45g/l. In the validating experiment, the ethanol fermentation rate reached 102.1g/l, closely matching the predicted rate. |
| 学科主题 | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | RESPONSE-SURFACE METHODOLOGY ; SOLID-STATE MEDIUM ; INULIN HYDROLYSIS ; OPTIMIZATION ; BIOFUELS ; MARKERS ; SUGAR ; L. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000333880000017 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8562]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Jiangsu Prov Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China 3.Qingdao Univ Sci & Technol QUST, Inst Life Sci, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Y. C.,Long, X. H.,Liu, Z. P.,et al. Optimizing medium for producing ethanol from industrial crop Jerusalem artichoke by one- step fermentation and recombinant Saccharomyces cerevisiae[J]. PLANT BIOSYSTEMS,2014,148(1):118-126. |
| APA | Du, Y. C.,Long, X. H.,Liu, Z. P.,&Shao, H. B..(2014).Optimizing medium for producing ethanol from industrial crop Jerusalem artichoke by one- step fermentation and recombinant Saccharomyces cerevisiae.PLANT BIOSYSTEMS,148(1),118-126. |
| MLA | Du, Y. C.,et al."Optimizing medium for producing ethanol from industrial crop Jerusalem artichoke by one- step fermentation and recombinant Saccharomyces cerevisiae".PLANT BIOSYSTEMS 148.1(2014):118-126. |
入库方式： OAI收割
来源：烟台海岸带研究所
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