Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry
文献类型：期刊论文
| 作者 | Chen, Fujia1; Long, Xiaohua1; Liu, Zhaopu1; Shao, Hongbo2,3; Liu, Ling1 |
| 刊名 | SCIENTIFIC WORLD JOURNAL
 |
| 出版日期 | 2014 |
| 页码 | 568043 |
| 关键词 | CHLOROGENIC ACIDS ANTIOXIDANT PROPERTIES RAT PLASMA IDENTIFICATION HPLC CONSTITUENTS DAD EXTRACT FRUIT MS |
| ISSN号 | 1537-744X |
| 产权排序 | [Chen, Fujia; Long, Xiaohua; Liu, Zhaopu; Liu, Ling] Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China; [Shao, Hongbo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China; [Shao, Hongbo] Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China |
| 通讯作者 | Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China. shaohongbochu@126.com ; liuling@njau.edu.cn |
| 中文摘要 | Plant phenolics can have applications in pharmaceutical and other industries. To identify and quantify the phenolic compounds in Helianthus tuberosus leaves, qualitative analysis was performed by a reversed phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and quantitative analysis by HPLC. Ten chlorogenic acids (CGAs) were identified (3-o-caffeoylquinic acid, two isomers of caffeoylquinic acid, caffeic acid, p-coumaroyl-quinic acid, feruloylquinic acid, 3,4-dicaffeoyquinic acid, 3,5-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) by comparing their retention times, UV-Vis absorption spectra, and MS/MS spectra with standards. In addition, four other phenolic compounds, including caffeoyl glucopyranose, isorhamnetin glucoside, kaempferol glucuronide, and kaempferol-3-o-glucoside, were tentatively identified in Helianthus tuberosus leaves for the first time. The 3-o-caffeoylquinic acid (7.752mg/g DW), 4,5-dicaffeoylquinic acid (5.633mg/g DW), and 3,5-dicaffeoylquinic acid (4.900mg/g DW) were the major phenolic compounds in leaves of Helianthus tuberosus cultivar NanYu in maturity. The variations in phenolic concentrations and proportions in Helianthus tuberosus leaves were influenced by genotype and plant growth stage. Cultivar NanYu had the highest concentration of phenolic compounds, in particular 3-o-caffeoylquinic acid and 4,5-dicaffeoylquinic acid compared with the other genotypes (wild accession and QingYu). Considering various growth stages, the concentration of total phenolics in cultivar NanYu was higher at flowering stage (5.270 mg/g DW) than at budding and tuber swelling stages. Cultivar NanYu of Helianthus tuberosus is a potential source of natural phenolics that may play an important role in the development of pharmaceuticals. |
| 英文摘要 | Plant phenolics can have applications in pharmaceutical and other industries. To identify and quantify the phenolic compounds in Helianthus tuberosus leaves, qualitative analysis was performed by a reversed phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and quantitative analysis by HPLC. Ten chlorogenic acids (CGAs) were identified (3-o-caffeoylquinic acid, two isomers of caffeoylquinic acid, caffeic acid, p-coumaroyl-quinic acid, feruloylquinic acid, 3,4-dicaffeoyquinic acid, 3,5-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) by comparing their retention times, UV-Vis absorption spectra, and MS/MS spectra with standards. In addition, four other phenolic compounds, including caffeoyl glucopyranose, isorhamnetin glucoside, kaempferol glucuronide, and kaempferol-3-o-glucoside, were tentatively identified in Helianthus tuberosus leaves for the first time. The 3-o-caffeoylquinic acid (7.752mg/g DW), 4,5-dicaffeoylquinic acid (5.633mg/g DW), and 3,5-dicaffeoylquinic acid (4.900mg/g DW) were the major phenolic compounds in leaves of Helianthus tuberosus cultivar NanYu in maturity. The variations in phenolic concentrations and proportions in Helianthus tuberosus leaves were influenced by genotype and plant growth stage. Cultivar NanYu had the highest concentration of phenolic compounds, in particular 3-o-caffeoylquinic acid and 4,5-dicaffeoylquinic acid compared with the other genotypes (wild accession and QingYu). Considering various growth stages, the concentration of total phenolics in cultivar NanYu was higher at flowering stage (5.270 mg/g DW) than at budding and tuber swelling stages. Cultivar NanYu of Helianthus tuberosus is a potential source of natural phenolics that may play an important role in the development of pharmaceuticals. |
| 学科主题 | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | CHLOROGENIC ACIDS ; ANTIOXIDANT PROPERTIES ; RAT PLASMA ; IDENTIFICATION ; HPLC ; CONSTITUENTS ; DAD ; EXTRACT ; FRUIT ; MS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000343479100001 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8568]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China 3.Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Fujia,Long, Xiaohua,Liu, Zhaopu,et al. Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry[J]. SCIENTIFIC WORLD JOURNAL,2014:568043. |
| APA | Chen, Fujia,Long, Xiaohua,Liu, Zhaopu,Shao, Hongbo,&Liu, Ling.(2014).Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry.SCIENTIFIC WORLD JOURNAL,568043. |
| MLA | Chen, Fujia,et al."Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry".SCIENTIFIC WORLD JOURNAL (2014):568043. |
入库方式： OAI收割
来源：烟台海岸带研究所
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