中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)

文献类型：期刊论文

作者Wei, Lei1; Wang, Qing1; Ning, Xuanxuan2; Mu, Changkao3; Wang, Chunlin3; Cao, Ruiwen1,4; Wu, Huifeng1; Cong, Ming1; Li, Fei1; Ji, Chenglong1
刊名COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
出版日期2015-03-01
卷号13页码:16-23
关键词Ocean acidification Proteomics Metabolomics Crassostrea gigas
ISSN号1744-117X
产权排序[Wei, Lei; Wang, Qing; Cao, Ruiwen; Wu, Huifeng; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Ning, Xuanxuan] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [Mu, Changkao; Wang, Chunlin] Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China; [Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者Wu, HF (reprint author), Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
中文摘要Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO(2) condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO(2) could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO(2) probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C.gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. (C) 2014 Elsevier Inc. All rights reserved.
英文摘要Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO(2) condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO(2) could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO(2) probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C.gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. (C) 2014 Elsevier Inc. All rights reserved.
学科主题Biochemistry & Molecular Biology; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]LASER-DESORPTION/IONIZATION-TIME ; CLAM RUDITAPES-PHILIPPINARUM ; EARTHWORM EISENIA-FOETIDA ; SMOOTH-MUSCLE MYOSIN ; OCEAN ACIDIFICATION ; MYTILUS-GALLOPROVINCIALIS ; SACCOSTREA-GLOMERATA ; 2,2',4,4'-TETRABROMODIPHENYL ETHER ; MALATE-DEHYDROGENASES ; METAL CONTAMINATION
收录类别SCI
语种英语
WOS记录号WOS:000351032100003
源URL[http://ir.yic.ac.cn/handle/133337/8646]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, YIC, Shandong Prov Key Lab Coastal Zone Environm Proc, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
2.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
3.Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wei, Lei,Wang, Qing,Ning, Xuanxuan,et al. Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2015,13:16-23.
APA Wei, Lei.,Wang, Qing.,Ning, Xuanxuan.,Mu, Changkao.,Wang, Chunlin.,...&Zhao, Jianmin.(2015).Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,13,16-23.
MLA Wei, Lei,et al."Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO(2)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 13(2015):16-23.

入库方式： OAI收割

来源：烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。