Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine
文献类型：期刊论文
| 作者 | Qin, Wei ; Ding, Jiawang; Lei, Jiahong
|
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2015-02-09 |
| 卷号 | 858页码:60-65 |
| 关键词 | Potentiometry Polycation-sensitive membrane electrodes Protamine Heparin Current-controlled release Flow injection analysis |
| ISSN号 | 0003-2670 |
| 产权排序 | [Lei, Jiahong; Ding, Jiawang; Chen, Yan; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Lei, Jiahong; Ding, Jiawang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn |
| 中文摘要 | A flow injection system incorporated with a polycation-sensitive polymeric membrane electrode in the flow cell is proposed for potentiometric determination of heparin. An external current in nano-ampere scale is continuously applied across the polymeric membrane for controlled release of protamine from the inner filling solution to the sample solution, which makes the electrode membrane regenerate quickly after each measurement. The protamine released at membrane-sample interface is consumed by heparin injected into the flow cell via their strong electrostatic interaction, thus decreasing the measured potential, by which heparin can be detected. Under optimized conditions, a linear relationship between the potential peak height and the concentration of heparin in the sample solution can be obtained in the range of 0.1-2.0 U mL (1), and the detection limit is 0.06 U mL (1). The proposed potentiometric sensing system has been successfully applied to the determination of heparin in undiluted sheep whole blood. (C) 2014 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry, Analytical |
| 研究领域[WOS] | Instrument Developing Project of the Chinese Academy of Sciences [YZ201161] ; National Natural Science Foundation of China [41176081, 21207156] ; Taishan Scholar Program of Shandong Province |
| 关键词[WOS] | SENSITIVE MEMBRANE ELECTRODES ; MOLECULAR-WEIGHT HEPARIN ; POLYION SENSORS ; ELECTROCHEMICAL SENSOR ; RESPONSE MECHANISM ; WHOLE-BLOOD ; LABEL-FREE ; POLYANIONS ; SURGERY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348038600008 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8655]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_环境化学实验室 |
| 推荐引用方式 GB/T 7714 | Qin, Wei,Ding, Jiawang,Lei, Jiahong. Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine[J]. ANALYTICA CHIMICA ACTA,2015,858:60-65. |
| APA | Qin, Wei,Ding, Jiawang,&Lei, Jiahong.(2015).Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine.ANALYTICA CHIMICA ACTA,858,60-65. |
| MLA | Qin, Wei,et al."Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine".ANALYTICA CHIMICA ACTA 858(2015):60-65. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。