Electrochemical Evaluation of the Mechanism of Acetylcholinesterase Inhibition Based on an Electrodeposited Thin Film
文献类型：期刊论文
| 作者 | Zhang, Hongxia1,2,3; Ding, Jiawang1,2 ; Du, Dan4
|
| 刊名 | INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
 |
| 出版日期 | 2015-02-01 |
| 卷号 | 10期号:2页码:1632-1645 |
| 关键词 | acetylcholinesterase biosensor inhibition mechanism electrochemical electrodeposition |
| ISSN号 | 1452-3981 |
| 产权排序 | [Zhang, Hongxia; Ding, Jiawang] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; [Zhang, Hongxia; Ding, Jiawang] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; [Zhang, Hongxia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Du, Dan] Huazhong Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China |
| 通讯作者 | Ding, JW (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China. jwding@mail.ccnu.edu.cn |
| 中文摘要 | An interface embedded gold nanoparticles in sol-gel thin film was constructed by one-step electrochemical deposition. Acetylcholinesterase (AChE) was physically absorbed onto to the interface to form a thin enzymatic layer. The proposed thin enzymatic layer, having kinetics similar to that of the enzyme in solution, provides an ideal sensing platform to electrochemically evaluate the chemical mechanism of enzyme inhibition. Lineweaver-Burk plot and surface plasmon resonance confirmed that the inhibition of AChE by malathion followed an irreversible mechanism and was a mixed type of competitive and noncompetitive. On the contrary, the degrees of inhibition by Pb2+ and Fe3+ were independent of the incubation time and the AChE concentrations, showing the reversibility of the inhibition. Furthermore, UV-vis absorption spectra indicated that the AChE mediated the hydrolysis of acetylthiocholine to yield a reducing agent thiocholine that reduced Fe3+ to Fe2+ and Fe2+ presented an effect of activation. To meet the demand of the biosensor design, we further investigated the relationship between inhibition percentage and both incubation time and inhibitor concentration. The enzyme's sensitivity to solvent effects and reactivation of the biosensor were also evaluated. It is anticipated that a rapid evaluation of the chemical mechanism of AChE inhibition could paves the way to rationally design biosensors and new compounds, as candidates for the treatment of Alzheimer's disease and pesticides. |
| 英文摘要 | An interface embedded gold nanoparticles in sol-gel thin film was constructed by one-step electrochemical deposition. Acetylcholinesterase (AChE) was physically absorbed onto to the interface to form a thin enzymatic layer. The proposed thin enzymatic layer, having kinetics similar to that of the enzyme in solution, provides an ideal sensing platform to electrochemically evaluate the chemical mechanism of enzyme inhibition. Lineweaver-Burk plot and surface plasmon resonance confirmed that the inhibition of AChE by malathion followed an irreversible mechanism and was a mixed type of competitive and noncompetitive. On the contrary, the degrees of inhibition by Pb2+ and Fe3+ were independent of the incubation time and the AChE concentrations, showing the reversibility of the inhibition. Furthermore, UV-vis absorption spectra indicated that the AChE mediated the hydrolysis of acetylthiocholine to yield a reducing agent thiocholine that reduced Fe3+ to Fe2+ and Fe2+ presented an effect of activation. To meet the demand of the biosensor design, we further investigated the relationship between inhibition percentage and both incubation time and inhibitor concentration. The enzyme's sensitivity to solvent effects and reactivation of the biosensor were also evaluated. It is anticipated that a rapid evaluation of the chemical mechanism of AChE inhibition could paves the way to rationally design biosensors and new compounds, as candidates for the treatment of Alzheimer's disease and pesticides. |
| 学科主题 | Electrochemistry |
| 研究领域[WOS] | Electrochemistry |
| 关键词[WOS] | GOLD NANOPARTICLES ; IMMOBILIZED ACETYLCHOLINESTERASE ; ORGANOPHOSPHOROUS INSECTICIDE ; AMPEROMETRIC DETECTION ; AU NANOPARTICLES ; BIOSENSOR DESIGN ; SILICATE FILMS ; CHITOSAN ; CARBON ; THIOCHOLINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000348365800047 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8658]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China 2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Huazhong Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hongxia,Ding, Jiawang,Du, Dan. Electrochemical Evaluation of the Mechanism of Acetylcholinesterase Inhibition Based on an Electrodeposited Thin Film[J]. INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE,2015,10(2):1632-1645. |
| APA | Zhang, Hongxia,Ding, Jiawang,&Du, Dan.(2015).Electrochemical Evaluation of the Mechanism of Acetylcholinesterase Inhibition Based on an Electrodeposited Thin Film.INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE,10(2),1632-1645. |
| MLA | Zhang, Hongxia,et al."Electrochemical Evaluation of the Mechanism of Acetylcholinesterase Inhibition Based on an Electrodeposited Thin Film".INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE 10.2(2015):1632-1645. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。