中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
Potentiometric Aptasensing of Listeria monocytogenes Using Protamine as an Indicator

文献类型：期刊论文

作者Ding, Jiawang1,2,3; Lei, Jiahong1,2,3; Ma, Xia1,2; Gong, Jun1,2; Qin, Wei1,2
刊名ANALYTICAL CHEMISTRY
出版日期2014-10-07
卷号86期号:19页码:9412-9416
关键词SENSITIVE MEMBRANE ELECTRODES FOODBORNE PATHOGENS SALMONELLA-TYPHIMURIUM APTAMER BACTERIA SELECTION BIOSENSOR SAMPLES IDENTIFICATION FLUORESCENCE
ISSN号0003-2700
产权排序[Ding, Jiawang; Lei, Jiahong; Ma, Xia; Gong, Jun; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang; Lei, Jiahong; Ma, Xia; Gong, Jun; Qin, Wei] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang; Lei, Jiahong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
中文摘要Exposure to pathogens in recreational or drinking water is a serious public health concern. It is important to rapidly determine and identify trace levels of pathogens in real environmental samples. We report here on a label-free potentiometric aptasensor for rapid, sensitive, and selective detection of Listeria monocytogenes (LM), a pathogen widely distributed in the environment. An aptamer binds specifically to internalin A, a surface protein present in LM cells. The target-binding event prevents the aptamer from electrostatically interacting with protamine, which can be sensitively detected using a polycation-sensitive membrane electrode. Using this method, LM can be detected down to 10 CFU mL(-1). Coupled to an online filtration system, the bioassay has been evaluated with spiked coastal seawater samples and shows good recovery and high accuracy. This work demonstrates the possibility of developing potentiometric aptasensors for determination and identification of various bacteria in environmental samples.
英文摘要Exposure to pathogens in recreational or drinking water is a serious public health concern. It is important to rapidly determine and identify trace levels of pathogens in real environmental samples. We report here on a label-free potentiometric aptasensor for rapid, sensitive, and selective detection of Listeria monocytogenes (LM), a pathogen widely distributed in the environment. An aptamer binds specifically to internalin A, a surface protein present in LM cells. The target-binding event prevents the aptamer from electrostatically interacting with protamine, which can be sensitively detected using a polycation-sensitive membrane electrode. Using this method, LM can be detected down to 10 CFU mL(-1). Coupled to an online filtration system, the bioassay has been evaluated with spiked coastal seawater samples and shows good recovery and high accuracy. This work demonstrates the possibility of developing potentiometric aptasensors for determination and identification of various bacteria in environmental samples.
学科主题Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]SENSITIVE MEMBRANE ELECTRODES ; FOODBORNE PATHOGENS ; SALMONELLA-TYPHIMURIUM ; APTAMER ; BACTERIA ; SELECTION ; BIOSENSOR ; SAMPLES ; IDENTIFICATION ; FLUORESCENCE
收录类别SCI
语种英语
WOS记录号WOS:000343017100013
源URL[http://ir.yic.ac.cn/handle/133337/8702]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_环境化学实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Ding, Jiawang,Lei, Jiahong,Ma, Xia,et al. Potentiometric Aptasensing of Listeria monocytogenes Using Protamine as an Indicator[J]. ANALYTICAL CHEMISTRY,2014,86(19):9412-9416.
APA Ding, Jiawang,Lei, Jiahong,Ma, Xia,Gong, Jun,&Qin, Wei.(2014).Potentiometric Aptasensing of Listeria monocytogenes Using Protamine as an Indicator.ANALYTICAL CHEMISTRY,86(19),9412-9416.
MLA Ding, Jiawang,et al."Potentiometric Aptasensing of Listeria monocytogenes Using Protamine as an Indicator".ANALYTICAL CHEMISTRY 86.19(2014):9412-9416.

入库方式： OAI收割

来源：烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。