Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal
文献类型：期刊论文
| 作者 | Zhang, Wei Wei1; Andong, Zheng Yang2; Zhang, Min3; Wang, Qiao Ning1; Wei, Ying Qin4; Chen, Ling Xin1
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| 刊名 | ANNALS OF MICROBIOLOGY
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| 出版日期 | 2014-09-01 |
| 卷号 | 64期号:3页码:1231-1238 |
| 关键词 | Ammonia Proteus mirabilis NH4+-N removal Ammonia monooxygenase mRNA level |
| ISSN号 | 1590-4261 |
| 产权排序 | [Zhang, Wei Wei; Wang, Qiao Ning; Chen, Ling Xin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Andong, Zheng Yang] China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Shandong, Peoples R China; [Zhang, Min] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China; [Wei, Ying Qin] Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan 250353, Shandong, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R China. wwzhang@yic.ac.cn ; Andongzhengyang123@163.com ; zhangmin@qdio.ac.cn ; wangqiaoning333@hotmail.com ; wyq@qlu.edu.cn ; lxchen@yic.ac.cn |
| 中文摘要 | Ammonia (referring to both NH3 and NH4 (+)), is one of the most toxic nitrogen forms, and is toxic to many aquatic organisms. The present study identified a heterotrophic nitrifier Proteus mirabilis strain V7, isolated from the coastal seawater. Almost 100 % of 82 mg L-1 NH4 (+)-N was removed within 48 h, while 51.2 % of total inorganic nitrogen was removed within 96 h after inoculation of Proteus mirabilis strain V7. Moreover, Proteus mirabilis strain V7 could remove NH4 (+)-N in a seawater matrix, but with much lower efficiency. Three water samples containing NH4 (+)-N at concentrations of 64.40, 18.00 and 9.82 mg L-1 were chosen to further test the NH4 (+)-N removal ability of Proteus mirabilis strain V7. All of the NH4 (+)-N was removed, with only a trace production of NO2 (-) and NO3 (-). A putative subunit of ammonia monooxygenase from Proteus mirabilis strain V7, amoA (Pm), was cloned and its mRNA level in the presence of NH4 (+) was characterized by real time reverse transcriptase PCR (RT-PCR). A 1.8-fold increase in the mRNA level of amoA (Pm) was observed, which indicated that AmoA may be involved in NH4 (+) oxidization. Our results indicated that Proteus mirabilis strain V7 could potentially be used as new resource for the treatment of NH4 (+)-N contaminated environments. |
| 英文摘要 | Ammonia (referring to both NH3 and NH4 (+)), is one of the most toxic nitrogen forms, and is toxic to many aquatic organisms. The present study identified a heterotrophic nitrifier Proteus mirabilis strain V7, isolated from the coastal seawater. Almost 100 % of 82 mg L-1 NH4 (+)-N was removed within 48 h, while 51.2 % of total inorganic nitrogen was removed within 96 h after inoculation of Proteus mirabilis strain V7. Moreover, Proteus mirabilis strain V7 could remove NH4 (+)-N in a seawater matrix, but with much lower efficiency. Three water samples containing NH4 (+)-N at concentrations of 64.40, 18.00 and 9.82 mg L-1 were chosen to further test the NH4 (+)-N removal ability of Proteus mirabilis strain V7. All of the NH4 (+)-N was removed, with only a trace production of NO2 (-) and NO3 (-). A putative subunit of ammonia monooxygenase from Proteus mirabilis strain V7, amoA (Pm), was cloned and its mRNA level in the presence of NH4 (+) was characterized by real time reverse transcriptase PCR (RT-PCR). A 1.8-fold increase in the mRNA level of amoA (Pm) was observed, which indicated that AmoA may be involved in NH4 (+) oxidization. Our results indicated that Proteus mirabilis strain V7 could potentially be used as new resource for the treatment of NH4 (+)-N contaminated environments. |
| 学科主题 | Biotechnology & Applied Microbiology; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | NITRIFYING-DENITRIFYING BACTERIUM ; AEROBIC DENITRIFICATION ; THIOSPHAERA-PANTOTROPHA ; AMMONIA MONOOXYGENASE ; NITROSOMONAS-EUROPAEA ; HYDROXYLAMINE OXIDOREDUCTASE ; SIMULTANEOUS NITRIFICATION ; ALCALIGENES-FAECALIS ; NITROGEN REMOVAL ; WASTE-WATER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340087200037 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8713]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 2.China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 4.Qilu Univ Technol, Sch Chem & Pharmaceut Engn, Jinan 250353, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei Wei,Andong, Zheng Yang,Zhang, Min,et al. Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal[J]. ANNALS OF MICROBIOLOGY,2014,64(3):1231-1238. |
| APA | Zhang, Wei Wei,Andong, Zheng Yang,Zhang, Min,Wang, Qiao Ning,Wei, Ying Qin,&Chen, Ling Xin.(2014).Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal.ANNALS OF MICROBIOLOGY,64(3),1231-1238. |
| MLA | Zhang, Wei Wei,et al."Isolation and characterization of a heterotrophic nitrifier Proteus mirabilis strain V7 and its potential application in NH4 (+)-N removal".ANNALS OF MICROBIOLOGY 64.3(2014):1231-1238. |
入库方式： OAI收割
来源：烟台海岸带研究所
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