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Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge

文献类型：期刊论文

作者Liu, Xiaoli1; Ji, Chenglong2; Zhao, Jianmin2; Wang, Qing2; Li, Fei2; Wu, Huifeng2
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2014-08-01
卷号39期号:2页码:372-377
关键词Metabolomics Mytilus galloprovincialis Digestive gland Gill Vibrio harveyi
ISSN号1050-4648
产权排序[Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Ji, Chenglong; Zhao, Jianmin; Wang, Qing; Li, Fei; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Coastal Ecotoxicol, Yantai 264003, Peoples R China
通讯作者Liu, XL (reprint author), Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China. zlylx12009@gmail.com
中文摘要Mussel Mytilus galloprovincialis is a marine aquaculture shellfish distributing widely along the coast in north China. In this work, we studied the differential metabolic responses induced by Vibrio harveyi in digestive gland and gill tissues from M. galloprovincialis using NMR-based metabolomics. The differential metabolic responses in the two tissue types were detected, except the similarly altered taurine and betaine. These metabolic responses suggested that V. harveyi mainly induced osmotic disruption and reduced energy demand via the metabolic pathways of glucose synthesis and ATP/AMP conversion in mussel digestive gland. In mussel gill tissues, V. harveyi basically caused osmotic stress and possible reduced energy demand as shown by the elevated phosphocholine that is involved in one of the metabolic pathways of ATP synthesis from ADP and phosphocholine. The altered mRNA expression levels of related genes (superoxide dismutase with copper and zinc, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear oxidative and immune stresses in both digestive gland and gill tissues. However, the mRNA expression levels of both lysozyme and defensin in digestive gland were more significantly up-regulated than those in gill from V harveyi-challenged mussel M. galloprovincialis, meaning that the immune organ, digestive gland, was more sensitive than gill. Overall, our results indicated that V. harveyi could induce tissue-specific metabolic responses in mussel M. galloprovincialis. (C) 2014 Elsevier Ltd. All rights reserved.
英文摘要Mussel Mytilus galloprovincialis is a marine aquaculture shellfish distributing widely along the coast in north China. In this work, we studied the differential metabolic responses induced by Vibrio harveyi in digestive gland and gill tissues from M. galloprovincialis using NMR-based metabolomics. The differential metabolic responses in the two tissue types were detected, except the similarly altered taurine and betaine. These metabolic responses suggested that V. harveyi mainly induced osmotic disruption and reduced energy demand via the metabolic pathways of glucose synthesis and ATP/AMP conversion in mussel digestive gland. In mussel gill tissues, V. harveyi basically caused osmotic stress and possible reduced energy demand as shown by the elevated phosphocholine that is involved in one of the metabolic pathways of ATP synthesis from ADP and phosphocholine. The altered mRNA expression levels of related genes (superoxide dismutase with copper and zinc, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear oxidative and immune stresses in both digestive gland and gill tissues. However, the mRNA expression levels of both lysozyme and defensin in digestive gland were more significantly up-regulated than those in gill from V harveyi-challenged mussel M. galloprovincialis, meaning that the immune organ, digestive gland, was more sensitive than gill. Overall, our results indicated that V. harveyi could induce tissue-specific metabolic responses in mussel M. galloprovincialis. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
研究领域[WOS]Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]CLAM RUDITAPES-PHILIPPINARUM ; GENE-EXPRESSION ; MEDITERRANEAN MUSSEL ; PATTERN-RECOGNITION ; NMR METABOLOMICS ; ANGUILLARUM ; HEMOCYTES ; PATHOGEN ; SPECTROSCOPY ; LA(NO3)(3)
收录类别SCI
语种英语
WOS记录号WOS:000339773000032
源URL[http://ir.yic.ac.cn/handle/133337/8723]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Lab Coastal Ecotoxicol, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Xiaoli,Ji, Chenglong,Zhao, Jianmin,et al. Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(2):372-377.
APA Liu, Xiaoli,Ji, Chenglong,Zhao, Jianmin,Wang, Qing,Li, Fei,&Wu, Huifeng.(2014).Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge.FISH & SHELLFISH IMMUNOLOGY,39(2),372-377.
MLA Liu, Xiaoli,et al."Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge".FISH & SHELLFISH IMMUNOLOGY 39.2(2014):372-377.

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来源：烟台海岸带研究所

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