Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design
文献类型：期刊论文
| 作者 | Qin, Wei ; Ding, Jiawang
|
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2014-08 |
| 卷号 | 199页码:284-290 |
| 关键词 | Nanoporous gold Carbon nanotubes Amperometry Acetylcholinesterase Pesticides |
| ISSN号 | 0925-4005 |
| 产权排序 | [Ding, Jiawang; Zhang, Hongxia; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, YICCAS, Yantai Inst Coastal Zone Res YIC,Key Lab Coastal, Yantai 264003, Shandong, Peoples R China; [Jia, Falong; Du, Dan] Huazhong Normal Univ, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China |
| 通讯作者 | Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, YICCAS, Yantai Inst Coastal Zone Res YIC,Key Lab Coastal, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn ; dan.du@mail.ccnu.edu.cn |
| 中文摘要 | An electrochemical sensing platform based on assembly of carbon nanotubes on a nanoporous gold electrode is described for highly sensitive detection of organophosphate pesticides. The nanoporous gold film (NPG) electrode is fabricated by an alloying/dealloying process, which possess high electroactive surface area and is an excellent substrate for sensor design. The NPG functionalized with cysteamine allows the immobilization of carbon nanotubes on the electrode with the self-assembly technique. The carboxylated carbon nanotubes are further linkered with acetylcholinesterase (AChE) for amperometric sensing of pesticides. The immobilized AChE, as a model, shows excellent activity to its substrate and allows a quantitative measurement of organophosphate pesticides. Under the optimal experimental conditions, the inhibition of malathion is proportional to its concentration in the range of 0.001-0.5 mu g mL(-1) with a detection limit of 0.5 ng mL(-1). The proposed method shows good reproducibility and high stability, which provides a new avenue for electrochemical biosensor design. (C) 2014 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation |
| 研究领域[WOS] | National Natural Science Foundation of China [21207156] ; Science and Technology Project of Yantai [2012132] ; Taishan Scholar Program of Shandong Province [TS20081159] |
| 关键词[WOS] | RAT-BRAIN ACETYLCHOLINESTERASE ; LINKED CHITOSAN COMPOSITE ; MODIFIED AU ELECTRODE ; ORGANOPHOSPHATE PESTICIDES ; ELECTROCHEMICAL PROPERTIES ; FILM ELECTRODES ; SURFACE-AREA ; POROUS GOLD ; NANOPARTICLES ; IMMOBILIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336878000040 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8724]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Qin, Wei |
| 推荐引用方式 GB/T 7714 | Qin, Wei,Ding, Jiawang. Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,199:284-290. |
| APA | Qin, Wei,&Ding, Jiawang.(2014).Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design.SENSORS AND ACTUATORS B-CHEMICAL,199,284-290. |
| MLA | Qin, Wei,et al."Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design".SENSORS AND ACTUATORS B-CHEMICAL 199(2014):284-290. |
入库方式： OAI收割
来源：烟台海岸带研究所
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