中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
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  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 中科院海岸带环境过程与生态修复重点实验室
Identification and mRNA expression of two 17 beta-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals

文献类型：期刊论文

作者Zhang, Yingying1,3; Wang, Qing1; Ji, Yinglu2; Zhang, Qian4; Wu, Huifeng1; Xie, Jia1,3; Zhao, Jianmin1
刊名ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
出版日期2014-05-01
卷号37期号:3页码:1243-1255
关键词17 beta-Hydroxysteroid dehydrogenases Mytilus galloprovincialis Digestive glands Endocrine disrupting chemicals Gene expression
ISSN号1382-6689
产权排序[Zhang, Yingying; Wang, Qing; Wu, Huifeng; Xie, Jia; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Re, Yantai 264003, Peoples R China; [Ji, Yinglu] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; [Zhang, Yingying; Xie, Jia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Qian] China Agr Univ, Yantai 264670, Peoples R China
通讯作者Zhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Re, Yantai 264003, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
中文摘要17 beta-Hydroxysteroid dehydrogenases (17 beta-HSDs) are multifunctional enzymes involved in the metabolism of steroids, fatty acids, retinoids and bile acid. In this study, two novel types of 17 beta-HSDs (named as MgHsd17b10 and MgHsd17b12) were cloned from Mytilus galloprovincialis by using rapid amplification of cDNA ends (RACE) approaches. Sequence analysis showed that MgHsd17b10 and MgHsd17b12 encoded a polypeptide of 259 and 325 amino acids, respectively. Phylogenetic analysis revealed that MgHsd17b10 and MgHsd17b12 were evolutionarily clustered with other invertebrate 17 beta-HSD type 10 and 17 beta-HSD type 12 homologues. The MgHsd17b10 and MgHsd17b12 transcripts could be detected in all examined tissues with higher expression levels in digestive glands and gonad. After exposed to endocrine disrupting chemicals (Bisphenol A or 2,2',4,4'-tetrabromodiphenyl ether), the expression of MgHsd17b10 and MgHsd17b12 transcripts was both down-regulated in digestive glands. These findings suggest that MgHsd17b10 and MgHsd17b12 perhaps play an important role in the endocrine regulation of M. galloprovincialis. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要17 beta-Hydroxysteroid dehydrogenases (17 beta-HSDs) are multifunctional enzymes involved in the metabolism of steroids, fatty acids, retinoids and bile acid. In this study, two novel types of 17 beta-HSDs (named as MgHsd17b10 and MgHsd17b12) were cloned from Mytilus galloprovincialis by using rapid amplification of cDNA ends (RACE) approaches. Sequence analysis showed that MgHsd17b10 and MgHsd17b12 encoded a polypeptide of 259 and 325 amino acids, respectively. Phylogenetic analysis revealed that MgHsd17b10 and MgHsd17b12 were evolutionarily clustered with other invertebrate 17 beta-HSD type 10 and 17 beta-HSD type 12 homologues. The MgHsd17b10 and MgHsd17b12 transcripts could be detected in all examined tissues with higher expression levels in digestive glands and gonad. After exposed to endocrine disrupting chemicals (Bisphenol A or 2,2',4,4'-tetrabromodiphenyl ether), the expression of MgHsd17b10 and MgHsd17b12 transcripts was both down-regulated in digestive glands. These findings suggest that MgHsd17b10 and MgHsd17b12 perhaps play an important role in the endocrine regulation of M. galloprovincialis. (C) 2014 Elsevier B.V. All rights reserved.
学科主题Environmental Sciences; Pharmacology & Pharmacy; Toxicology
研究领域[WOS]Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology
关键词[WOS]CHROMATOGRAPHY-MASS SPECTROMETRY ; POLYBROMINATED DIPHENYL ETHERS ; ESTROGEN-RECEPTOR-ALPHA ; BISPHENOL-A ; IN-VITRO ; HYDROXYSTEROID DEHYDROGENASES ; DIALLYL PHTHALATE ; CLONING ; VITELLOGENIN ; BETA
收录类别SCI
语种英语
WOS记录号WOS:000338598200040
源URL[http://ir.yic.ac.cn/handle/133337/8749]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Re, Yantai 264003, Peoples R China
2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.China Agr Univ, Yantai 264670, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Yingying,Wang, Qing,Ji, Yinglu,et al. Identification and mRNA expression of two 17 beta-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2014,37(3):1243-1255.
APA Zhang, Yingying.,Wang, Qing.,Ji, Yinglu.,Zhang, Qian.,Wu, Huifeng.,...&Zhao, Jianmin.(2014).Identification and mRNA expression of two 17 beta-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,37(3),1243-1255.
MLA Zhang, Yingying,et al."Identification and mRNA expression of two 17 beta-hydroxysteroid dehydrogenase genes in the marine mussel Mytilus galloprovincialis following exposure to endocrine disrupting chemicals".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 37.3(2014):1243-1255.

入库方式： OAI收割

来源：烟台海岸带研究所

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