Molecular characterization of two glutathione peroxidase genes in Mytilus galloprovincialis and their transcriptional responses to sub-chronic arsenate and cadmium exposure
文献类型：期刊论文
| 作者 | Wang, Q.1 ; Ning, X.2; Zhang, Q.3; Liu, F.3; Wu, H.1; Zhang, Y.1; Zhao, J.1
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| 刊名 | ISJ-INVERTEBRATE SURVIVAL JOURNAL
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| 出版日期 | 2014 |
| 卷号 | 11页码:149-162 |
| 关键词 | cytosolic GPx phospholipid-hydroperoxide GPx arsenic cadmium bivalve antioxidant enzyme |
| ISSN号 | 1824-307X |
| 产权排序 | [Wang, Q.; Wu, H.; Zhang, Y.; Zhao, J.] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Ning, X.] SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China; [Zhang, Q.; Liu, F.] China Agr Univ Yantai, Yantai 264670, Peoples R China |
| 通讯作者 | Wu, H (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn |
| 中文摘要 | Glutathione peroxidases (GPxs) are key enzymes in the antioxidant defense system of living organisms, and protect organisms against oxidative stresses. In this study, the full-length cDNA sequences encoding cytosolic GPx (MgcGPx) and phospholipid-hydroperoxide GPx (MgGPx4) were identified from Mytilus galloprovincialis. The mussels were exposed to 0, 1, 10, and 100 mu g/L cadmium and arsenate for 30 days. The mRNA transcripts of these two genes and total GPx activity were examined in the gills and digestive gland after contaminants exposure. The mussels exposed to cadmium and arsenate responded mainly by down-regulating MgcGPx and MgGPx4 mRNA transcription in gills and up-regulating transcription in digestive gland. However, total GPx activities increased following cadmium exposure but decreased after arsenate stress in both tissues. These results suggest that MgcGPx and MgGPx4 perhaps play an important role in maintaining cellular redox homeostasis and protecting M. galloprovincialis against cadmium and arsenate toxicity. It can also be inferred that these genes have the potential to be used as molecular biomarkers for assessing cellular stress and toxicity of contaminants in this mussel. |
| 英文摘要 | Glutathione peroxidases (GPxs) are key enzymes in the antioxidant defense system of living organisms, and protect organisms against oxidative stresses. In this study, the full-length cDNA sequences encoding cytosolic GPx (MgcGPx) and phospholipid-hydroperoxide GPx (MgGPx4) were identified from Mytilus galloprovincialis. The mussels were exposed to 0, 1, 10, and 100 mu g/L cadmium and arsenate for 30 days. The mRNA transcripts of these two genes and total GPx activity were examined in the gills and digestive gland after contaminants exposure. The mussels exposed to cadmium and arsenate responded mainly by down-regulating MgcGPx and MgGPx4 mRNA transcription in gills and up-regulating transcription in digestive gland. However, total GPx activities increased following cadmium exposure but decreased after arsenate stress in both tissues. These results suggest that MgcGPx and MgGPx4 perhaps play an important role in maintaining cellular redox homeostasis and protecting M. galloprovincialis against cadmium and arsenate toxicity. It can also be inferred that these genes have the potential to be used as molecular biomarkers for assessing cellular stress and toxicity of contaminants in this mussel. |
| 学科主题 | Immunology; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | OXIDATIVE STRESS ; LIPID-PEROXIDATION ; VENERUPIS-PHILIPPINARUM ; SIGNAL-TRANSDUCTION ; EXPRESSION ANALYSIS ; AQUATIC ORGANISMS ; UNIO-TUMIDUS ; ANTIOXIDANT ; BIOMARKERS ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000350915300013 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8773]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_近岸生态与环境实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 3.China Agr Univ Yantai, Yantai 264670, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Q.,Ning, X.,Zhang, Q.,et al. Molecular characterization of two glutathione peroxidase genes in Mytilus galloprovincialis and their transcriptional responses to sub-chronic arsenate and cadmium exposure[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2014,11:149-162. |
| APA | Wang, Q..,Ning, X..,Zhang, Q..,Liu, F..,Wu, H..,...&Zhao, J..(2014).Molecular characterization of two glutathione peroxidase genes in Mytilus galloprovincialis and their transcriptional responses to sub-chronic arsenate and cadmium exposure.ISJ-INVERTEBRATE SURVIVAL JOURNAL,11,149-162. |
| MLA | Wang, Q.,et al."Molecular characterization of two glutathione peroxidase genes in Mytilus galloprovincialis and their transcriptional responses to sub-chronic arsenate and cadmium exposure".ISJ-INVERTEBRATE SURVIVAL JOURNAL 11(2014):149-162. |
入库方式： OAI收割
来源：烟台海岸带研究所
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