Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin
文献类型：期刊论文
| 作者 | Zhang, Zhong1,3; Li, Jinhua1 ; Fu, Junqing1,2; Chen, Lingxin1
|
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2014 |
| 卷号 | 4期号:40页码:20677-20685 |
| 关键词 | SOLID-PHASE EXTRACTION DRUG-DELIVERY C-PHYCOCYANIN POLYMERS PROTEIN NANOPARTICLES SURFACE SEPARATION SAMPLES |
| ISSN号 | 2046-2069 |
| 产权排序 | [Zhang, Zhong; Li, Jinhua; Fu, Junqing; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Fu, Junqing] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China; [Zhang, Zhong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
| 中文摘要 | Molecular imprinting as a versatile technology is emerging for diverse species in various fields; however protein imprinting faces several problems related to the size, structural complexity, conformational flexibility, and compatibility with solvents. Herein, by using phycocyanin as a model, with physiological significance and fluorescence characteristics, we developed a facile and highly efficient approach to obtain fluorescent and magnetic dual-responsive coreshell imprinting microspheres. Twostage miniemulsion polymerization was employed, based on surface immobilization of phycocyanin with aminolysis and aldehyde modification on superparamagnetic support particles. The dual-responsive imprinting microspheres exhibited high adsorption capacity of 10.53 mg g(-1), excellent binding selectivity toward phycocyanin with a high imprinting factor of 2.41, and good reproducibility with standard error within 10%. Furthermore, fast simple magnetic separation and sensitive fluorescent detection in a wide pH range was offered for phycocyanin, showing a good linearity within 0.01-1.0 mg L-1 (R-2 = 0.9970) and a favorable detectability up to 1.5 ng mL(-1). Consequently, the imprinting microspheres were successfully applied as sorbents for selective isolation of phycocyanin from protein mixtures and special imaging recognition. Taking advantages of dual-responsive polymers and surface imprinting, the developed strategy provides great application potentials for convenient, rapid targeting identification/enrichment and separation of proteins and thereby contributing to targeting drug delivery and protein research. |
| 英文摘要 | Molecular imprinting as a versatile technology is emerging for diverse species in various fields; however protein imprinting faces several problems related to the size, structural complexity, conformational flexibility, and compatibility with solvents. Herein, by using phycocyanin as a model, with physiological significance and fluorescence characteristics, we developed a facile and highly efficient approach to obtain fluorescent and magnetic dual-responsive coreshell imprinting microspheres. Twostage miniemulsion polymerization was employed, based on surface immobilization of phycocyanin with aminolysis and aldehyde modification on superparamagnetic support particles. The dual-responsive imprinting microspheres exhibited high adsorption capacity of 10.53 mg g(-1), excellent binding selectivity toward phycocyanin with a high imprinting factor of 2.41, and good reproducibility with standard error within 10%. Furthermore, fast simple magnetic separation and sensitive fluorescent detection in a wide pH range was offered for phycocyanin, showing a good linearity within 0.01-1.0 mg L-1 (R-2 = 0.9970) and a favorable detectability up to 1.5 ng mL(-1). Consequently, the imprinting microspheres were successfully applied as sorbents for selective isolation of phycocyanin from protein mixtures and special imaging recognition. Taking advantages of dual-responsive polymers and surface imprinting, the developed strategy provides great application potentials for convenient, rapid targeting identification/enrichment and separation of proteins and thereby contributing to targeting drug delivery and protein research. |
| 学科主题 | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SOLID-PHASE EXTRACTION ; DRUG-DELIVERY ; C-PHYCOCYANIN ; POLYMERS ; PROTEIN ; NANOPARTICLES ; SURFACE ; SEPARATION ; SAMPLES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336833400011 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8786]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhong,Li, Jinhua,Fu, Junqing,et al. Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin[J]. RSC ADVANCES,2014,4(40):20677-20685. |
| APA | Zhang, Zhong,Li, Jinhua,Fu, Junqing,&Chen, Lingxin.(2014).Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin.RSC ADVANCES,4(40),20677-20685. |
| MLA | Zhang, Zhong,et al."Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin".RSC ADVANCES 4.40(2014):20677-20685. |
入库方式： OAI收割
来源：烟台海岸带研究所
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