Purification and Characterization of Bioactive Compounds from Styela clava
文献类型：期刊论文
| 作者 | Ju, Bao1; Chen, Bin2; Zhang, Xingrong1; Han, Chunling1; Jiang, Aili1 |
| 刊名 | JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2014 |
| 页码 | 525141 |
| 关键词 | DIDEMNIN-B MARINE EXTRACTS TUNICATE AGENT |
| ISSN号 | 2090-9063 |
| 产权排序 | [Ju, Bao; Zhang, Xingrong; Han, Chunling; Jiang, Aili] Yantai Univ, Dept Bioengn, Yantai 264005, Shandong, Peoples R China; [Chen, Bin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China |
| 通讯作者 | Jiang, AL (reprint author), Yantai Univ, Dept Bioengn, Yantai 264005, Shandong, Peoples R China. jal9035@live.cn |
| 中文摘要 | The immunomodulatory activity of extract from Styela clava was studied systematically based on activity tracking in vitro in order to find out novel-structured secondary metabolite. The proliferation rates of mouse splenic lymphocytes and peritoneal macrophages were used as screening index, as well as NO release promoting activities. The crude extract (CE) and its different polar fractions from S. clava all exhibited proliferative activity of splenolymphocytes and mouse macrophages, as well as NO release promoting activities, among which petroleum ether fraction (PE) showed the strongest effect. The antioxidant experiment in vitro showed that CE demonstrated antioxidant ability in 1,1-diphenyl-2-picrylhydrazyl (DPPH) system and the beta carotene-linoleic acid system; the activity of ethyl acetate fraction (ET) was much stronger than that of the others. Further isolated by silica gel column chromatography, ET was classified into seven sub-components (E1 similar to E7) listed in the order of activity as E5 > E6 > E4 > E3 > E7 > E2 > E1. Five compounds were separated as (1) cholesteric-7-en-3 beta-ol, (2) cholesteric-4-en-3 beta,6 beta-diol, (3) cholesterol, (4) batilol, and (5) ceramide, among which (1), (2), and (4) were isolated for the first time from S. clava. |
| 英文摘要 | The immunomodulatory activity of extract from Styela clava was studied systematically based on activity tracking in vitro in order to find out novel-structured secondary metabolite. The proliferation rates of mouse splenic lymphocytes and peritoneal macrophages were used as screening index, as well as NO release promoting activities. The crude extract (CE) and its different polar fractions from S. clava all exhibited proliferative activity of splenolymphocytes and mouse macrophages, as well as NO release promoting activities, among which petroleum ether fraction (PE) showed the strongest effect. The antioxidant experiment in vitro showed that CE demonstrated antioxidant ability in 1,1-diphenyl-2-picrylhydrazyl (DPPH) system and the beta carotene-linoleic acid system; the activity of ethyl acetate fraction (ET) was much stronger than that of the others. Further isolated by silica gel column chromatography, ET was classified into seven sub-components (E1 similar to E7) listed in the order of activity as E5 > E6 > E4 > E3 > E7 > E2 > E1. Five compounds were separated as (1) cholesteric-7-en-3 beta-ol, (2) cholesteric-4-en-3 beta,6 beta-diol, (3) cholesterol, (4) batilol, and (5) ceramide, among which (1), (2), and (4) were isolated for the first time from S. clava. |
| 学科主题 | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | DIDEMNIN-B ; MARINE ; EXTRACTS ; TUNICATE ; AGENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335774000001 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8805]  |
| 专题 | 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 |
| 作者单位 | 1.Yantai Univ, Dept Bioengn, Yantai 264005, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ju, Bao,Chen, Bin,Zhang, Xingrong,et al. Purification and Characterization of Bioactive Compounds from Styela clava[J]. JOURNAL OF CHEMISTRY,2014:525141. |
| APA | Ju, Bao,Chen, Bin,Zhang, Xingrong,Han, Chunling,&Jiang, Aili.(2014).Purification and Characterization of Bioactive Compounds from Styela clava.JOURNAL OF CHEMISTRY,525141. |
| MLA | Ju, Bao,et al."Purification and Characterization of Bioactive Compounds from Styela clava".JOURNAL OF CHEMISTRY (2014):525141. |
入库方式： OAI收割
来源：烟台海岸带研究所
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