beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments
文献类型：期刊论文
| 作者 | Gao, Na1,2; Lu, Feng3; Xiao, Chuang1 ; Yang, Lian1; Chen, Jun1; Zhou, Kai1; Wen, Dandan4; Li, Zi1; Wu, Mingyi1; Jiang, Jianmin4
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| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2015-08-20 |
| 卷号 | 127页码:427-437 |
| 关键词 | Fucosylated chondroitin sulfate Benzyl esterification beta-Elimination Depolymerization Anticoagulant |
| 通讯作者 | Liu,GM (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,132 Lanhei Rd,Kunming 650201,Yunnan,Peoples R China. ; lgm13330555378@126.com ; zhao.jinhua@yahoo.com |
| 英文摘要 | Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for beta-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GaINAc-beta 1,4-GlcA were selectively cleaved and distinctive Delta(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with M-w 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. (C) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Chemistry, Applied; Chemistry, Organic; Polymer Science |
| 类目[WOS] | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Polymer Science |
| 关键词[WOS] | STICHOPUS-JAPONICUS SELENKA ; SEA-CUCUMBER ; BODY-WALL ; GLYCOSAMINOGLYCAN ; HEPARIN ; POLYSACCHARIDES ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000355707500053 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/21341]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Dali Univ, Coll Pharm & Chem, Dali, Peoples R China 4.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510275, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Na,Lu, Feng,Xiao, Chuang,et al. beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments[J]. CARBOHYDRATE POLYMERS,2015,127:427-437. |
| APA | Gao, Na.,Lu, Feng.,Xiao, Chuang.,Yang, Lian.,Chen, Jun.,...&Zhao, Jinhua.(2015).beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments.CARBOHYDRATE POLYMERS,127,427-437. |
| MLA | Gao, Na,et al."beta-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments".CARBOHYDRATE POLYMERS 127(2015):427-437. |
入库方式： OAI收割
来源：昆明植物研究所
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