Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative
文献类型：期刊论文
| 作者 | Xu,Xiongbo; Gu,Zixin; Liu,Shao; Gao,Na; He,Xiaozhen; Xin,Xiu |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2015-09-05 |
| 卷号 | 128页码:138-146 |
| 关键词 | BCG-PSN Purification Characterization Sulfated derivative Antitumor |
| 通讯作者 | Liu,S (reprint author),Xiangya Hosp,Dept Pharm,Changsha 410008,Hunan,Peoples R China. ; liushao999@hotmail.com |
| 英文摘要 | A water-soluble glucan, BCG-PASW, with a molecular weight of 2.10 x 10(4) Da, was separated from polysaccharide nucleic acid fraction of Bacillus Calmette Guerin (BCG-PSN) using DEAE-52 cellulose and Sephadex G-200 chromatography. Based on gas chromatography-mass spectrometry (GC-MS), fourier transform infrared (FT-IR) spectra, 1D and 2D nuclear magnetic resonance (NMR) spectroscopy techniques (COSY, HSQC and HMBC), BCG-PASW was found to be an alpha-D-glucan composed of alpha-D-(1 -> 4)-linked glucopyranosyl residues, with branches at O-6 consisting of non-reducing terminal alpha-D-Glcp approximately every eight residues. In vitro antitumor activity by MTS method, its sulfated derivative with a substitution degree of 0.59, could inhibite C666-1 nasopharyngeal carcinoma cells growth significantly. The results indicated that the sulfate content play a decisive role in the bioactivities of the polysaccharides. (C) 2015 Elsevier Ltd. All rights reserved. |
| 学科主题 | Chemistry, Applied; Chemistry, Organic; Polymer Science |
| 类目[WOS] | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| 研究领域[WOS] | Chemistry ; Polymer Science |
| 关键词[WOS] | MAGNETIC-RESONANCE SPECTROSCOPY ; ANTIOXIDANT ACTIVITY ; IMMUNOSTIMULATORY ACTIVITY ; STRUCTURAL FEATURES ; RADIX-HEDYSARI ; POLYSACCHARIDE ; ANTIGLYCATION ; AMYGDALIN ; SINENSIS ; ROOTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000356209900016 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/21358]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xu,Xiongbo,Gu,Zixin,Liu,Shao,et al. Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative[J]. CARBOHYDRATE POLYMERS,2015,128:138-146. |
| APA | Xu,Xiongbo,Gu,Zixin,Liu,Shao,Gao,Na,He,Xiaozhen,&Xin,Xiu.(2015).Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative.CARBOHYDRATE POLYMERS,128,138-146. |
| MLA | Xu,Xiongbo,et al."Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative".CARBOHYDRATE POLYMERS 128(2015):138-146. |
入库方式： OAI收割
来源：昆明植物研究所
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