Dynamics of sulfate reduction and sulfate-reducing prokaryotes in anaerobic paddy soil amended with rice straw
文献类型：期刊论文
| 作者 | He, Ji-Zheng; Liu, Xin-Zhan; Zheng, Yong; Shen, Ju-Pei; Zhang, Li-Mei |
| 刊名 | BIOLOGY AND FERTILITY OF SOILS
 |
| 出版日期 | 2010-03 |
| 卷号 | 46期号:3页码:283-291 |
| 关键词 | Sulfate-reducing prokaryotes Paddy soil Acetate Quantification Real-time PCR Community structure Clone libraries |
| 中文摘要 | Incorporation of rice straw to soil is a common agricultural practice in rice cultivation. In anaerobic paddy soil, the complete mineralization of organic matter to CH(4) and CO(2) is accomplished by the sequential reduction of nitrate, ferric iron, sulfate, and methanogenesis. In order to estimate the temporal changes of sulfate-reducing prokaryotes (SRP) as decomposers of organic matters, the effects of rice straw amendment on the dynamics of sulfate reduction and SRP were investigated by combining the monitoring of CH(4), sulfate, and organic acids with molecular tools such as soil DNA extraction, real-time PCR, cloning, sequencing, and phylogenetic analysis. The incorporation of rice straw into paddy soil significantly increased concentrations of sulfate, formate, acetate, propionate, and lactate and CH(4) production. The rate of sulfate reduction in the straw-amended slurries was significantly higher than that in the unamended slurries. The dsrAB gene copy numbers of SRP in the straw-amended soil slurries ranged from 4.26 x 10(6) to 1.96 x 10(8) per gram of dry soil, which were significantly higher than those in the unamended control ranging from 1.99 x 10(6) to 7.90 x 10(7) per gram of dry soil. Significant correlations were observed between SRP dsrAB gene copy numbers and the concentrations of sulfate and acetate. Cloning and sequencing analyses showed a clear shift of SRP community structure between treatments and time. In the straw-amended slurries, Clostridia-like SRP significantly increased, while Deltaproterobacteria-like SRP (Sytrophobacter, Desulfobacterium, Desulfovibrio, and Desulfomonile) decreased during the incubation period. Novel uncultured SRP were abundant in the straw-amended slurries and changed during the incubation period. |
| WOS记录号 | WOS:000274939800008 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/20961]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 推荐引用方式 GB/T 7714 | He, Ji-Zheng,Liu, Xin-Zhan,Zheng, Yong,et al. Dynamics of sulfate reduction and sulfate-reducing prokaryotes in anaerobic paddy soil amended with rice straw[J]. BIOLOGY AND FERTILITY OF SOILS,2010,46(3):283-291. |
| APA | He, Ji-Zheng,Liu, Xin-Zhan,Zheng, Yong,Shen, Ju-Pei,&Zhang, Li-Mei.(2010).Dynamics of sulfate reduction and sulfate-reducing prokaryotes in anaerobic paddy soil amended with rice straw.BIOLOGY AND FERTILITY OF SOILS,46(3),283-291. |
| MLA | He, Ji-Zheng,et al."Dynamics of sulfate reduction and sulfate-reducing prokaryotes in anaerobic paddy soil amended with rice straw".BIOLOGY AND FERTILITY OF SOILS 46.3(2010):283-291. |
入库方式： OAI收割
来源：生态环境研究中心
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