Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China
文献类型：期刊论文
| 作者 | Zhang, Fan ; She, Yuehui ; Zheng, Yong ; Zhou, Zhifeng ; Kong, Shuqiong ; Hou, Dujie |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2010-04 |
| 卷号 | 86期号:4页码:1183-1194 |
| 关键词 | Community structure Gas and oil field Methanotrophic pmoA gene Real-time PCR Clone library |
| 中文摘要 | Currently, molecular biologic techniques achieve a great development in studies of soil samples. The objective of this research is to improve methods for microbial prospecting of oil and gas by applying culture-independent techniques to soil sampled from above a known oil and gas field. Firstly, the community structure of soil bacteria above the Ban 876 Gas and Oil Field was analyzed based on 16S rRNA gene clone libraries. The soil bacteria communities were consistently different along the depth; however, Chloroflexi and Gemmatimonadetes were predominant and methanotrophs were minor in both bacteria libraries (DGS1 and DGS2). Secondly, the numbers of methane-oxidizing bacteria, quantified using a culture-dependent procedure and culture-independent group-specific real-time PCR (RT-PCR), respectively, were inconsistent with a quantify variance of one or two orders of magnitude. Special emphasis was given to the counting advantages of RT-PCR based on the methanotrophic pmoA gene. Finally, the diversity and distribution of methanotrophic communities in the soil samples were analyzed by constructing clone libraries of functional gene. All 508-bp inserts in clones phylogenetically belonged to the methanotrophic pmoA gene with similarities from 83% to 100%. However, most of the similarities were below 96%. Five clone libraries of methanotrophs clearly showed that the anomalous methanotrophs (Methylosinus and Methylocystis) occupy the studied area. |
| WOS记录号 | WOS:000275965700018 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/20997]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Fan,She, Yuehui,Zheng, Yong,et al. Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,86(4):1183-1194. |
| APA | Zhang, Fan,She, Yuehui,Zheng, Yong,Zhou, Zhifeng,Kong, Shuqiong,&Hou, Dujie.(2010).Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,86(4),1183-1194. |
| MLA | Zhang, Fan,et al."Molecular biologic techniques applied to the microbial prospecting of oil and gas in the Ban 876 gas and oil field in China".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 86.4(2010):1183-1194. |
入库方式： OAI收割
来源：生态环境研究中心
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