Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds
文献类型：期刊论文
| 作者 | Zhao Bin; Baston, David S.; Khan, Elaine; Sorrentino, Claudio; Denison, Michael S. |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2010-05 |
| 卷号 | 53期号:5页码:1010-1016 |
| 关键词 | dioxin reporter gene bioassay CALUX CAFLUX |
| 中文摘要 | Reporter genes produce a protein product in transfected cells that can be easily measured in intact or lysed cells and they have been extensively used in numerous basic and applied research applications. Over the past 10 years, reporter gene assays have been widely accepted and used for analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin and related dioxin-like compounds in various types of matrices, such as biological, environmental, food and feed samples, given that high-resolution instrumental analysis techniques are impractical for large-scale screening analysis. The most sensitive cell-based reporter gene bioassay systems developed are the mechanism-based CALUX (Chemically Activated Luciferase Expression) and CAFLUX (Chemically Activated Fluorescent Expression) bioassays, which utilize recombinant cell lines containing stably transfected dioxin (AhR)-responsive firefly luciferase or enhanced green fluorescent protein (EGFP) reporter genes, respectively. While the current CALUX and CAFLUX bioassays are very sensitive, increasing their lower limit of sensitivity, magnitude of response and dynamic range for chemical detection would significantly increase their utility, particularly for those samples that contain low levels of dioxin-like HAHs (i.e., serum). In this study, we report that the addition of modulators of cell signaling pathways or modification of cell culture conditions results in significant improvement in the magnitude and overall responsiveness of the existing CALUX and CAFLUX cell bioassays. |
| WOS记录号 | WOS:000278263500007 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21076]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhao Bin,Baston, David S.,Khan, Elaine,et al. Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds[J]. SCIENCE CHINA-CHEMISTRY,2010,53(5):1010-1016. |
| APA | Zhao Bin,Baston, David S.,Khan, Elaine,Sorrentino, Claudio,&Denison, Michael S..(2010).Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds.SCIENCE CHINA-CHEMISTRY,53(5),1010-1016. |
| MLA | Zhao Bin,et al."Enhancing the response of CALUX and CAFLUX cell bioassays for quantitative detection of dioxin-like compounds".SCIENCE CHINA-CHEMISTRY 53.5(2010):1010-1016. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。