Highly sensitive electrochemiluminescence displacement method for the study of DNA/small molecule binding interactions
文献类型：期刊论文
| 作者 | Huang, Rongfu; Wang, Li-Rong; Guo, Liang-Hong |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2010-08-31 |
| 卷号 | 676期号:3页码:41-45 |
| 关键词 | Electrochemiluminescence Displacement DNA Ligand Binding |
| 中文摘要 | Non-covalent binding interactions of small molecules with DNA play important roles in regulating gene expression and gene function. In this work, a highly sensitive electrochemiluminescence (ECL) displacement method has been developed to investigate such interactions, particularly for weak DNA binders. This ECL method relies on a double-stranded DNA film deposited on an indium tin oxide electrode (ITO) surface by layer-by-layer self-assembly. A DNA intercalator, [Ru(bpy)(2)(dppz)](2+) (bpy = 2,2'-bipyridine, dppz = dipyrido[3,2-a:2'3'-c]phenazine), is employed as the ECL signal indicator. If a test compound competes with the indicator for the same binding sites in DNA, it would displace the indicator from the film and reduce ECL signal. The new method was validated by measuring five well-known DNA-binding organic molecules including quinacrine, H33258, thiazole orange, ethidium bromide and 4,6-diamidine-2-phenylindole dihydrochloride. Due to high ECL sensitivity, only 0.4 mu mol L(-1) [Ru(bpy)(2)(dppz)](2+) was needed in the ECL displacement measurement, which is about 75-fold less than the concentration in the voltammetric measurement. The lowered concentration permitted direct measurement of IC(50) values of eight hydroxylated polycyclic aromatic hydrocarbons in their ECL displacement curves and subsequent calculation of their binding constants with DNA. The ECL displacement method is particularly useful for investigating weak DNA binders with limited aqueous solubility. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000281990500006 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21085]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Huang, Rongfu,Wang, Li-Rong,Guo, Liang-Hong. Highly sensitive electrochemiluminescence displacement method for the study of DNA/small molecule binding interactions[J]. ANALYTICA CHIMICA ACTA,2010,676(3):41-45. |
| APA | Huang, Rongfu,Wang, Li-Rong,&Guo, Liang-Hong.(2010).Highly sensitive electrochemiluminescence displacement method for the study of DNA/small molecule binding interactions.ANALYTICA CHIMICA ACTA,676(3),41-45. |
| MLA | Huang, Rongfu,et al."Highly sensitive electrochemiluminescence displacement method for the study of DNA/small molecule binding interactions".ANALYTICA CHIMICA ACTA 676.3(2010):41-45. |
入库方式： OAI收割
来源：生态环境研究中心
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