Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis
文献类型：期刊论文
| 作者 | Li ZhiYong; Zhang QianYun; Zhao LiXia; Li ZhenJia; Hu GuoMao; Lin Jin-Ming; Wang Shan |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2010-04 |
| 卷号 | 53期号:4页码:812-819 |
| 关键词 | micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker |
| 中文摘要 | A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in micro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H(2)O(2))-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL(-1). The detection limit of CEA was 0.61 ng mL(-1). The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation. |
| WOS记录号 | WOS:000278263400015 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21107]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li ZhiYong,Zhang QianYun,Zhao LiXia,et al. Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis[J]. SCIENCE CHINA-CHEMISTRY,2010,53(4):812-819. |
| APA | Li ZhiYong.,Zhang QianYun.,Zhao LiXia.,Li ZhenJia.,Hu GuoMao.,...&Wang Shan.(2010).Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis.SCIENCE CHINA-CHEMISTRY,53(4),812-819. |
| MLA | Li ZhiYong,et al."Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis".SCIENCE CHINA-CHEMISTRY 53.4(2010):812-819. |
入库方式： OAI收割
来源：生态环境研究中心
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