Micro-plate Magnetic Chemiluminescence Immunoassay of Carbohydrate Antigen 125 in Serum
文献类型：期刊论文
| 作者 | Li Zhiyong; Xiao Qin; Ying Xitang; Li Zhenjia; Lin Jinming |
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2010-01-28 |
| 卷号 | 68期号:2页码:162-168 |
| 关键词 | chemiluminescence immunoassay magnetic particle micro-plate carcinoembryonic antigen 125 |
| 中文摘要 | A micro-plate magnetic chemiluminescence immunoassay (MMCLIA) for the determination of human carbohydrate antigen 125 (CA125) was developed based on traditional micro-plate chemiluminescence immunoassay (micro-plate CLIA) and magnetic particle-based chemiluminescence immunoassay (MP-CLIA). This method used magnetic particles as solid support and the micro-plate as a reactor. The horseradish peroxidase (HRP)-luminol-H(2)O(2) Chemiluminescent system was chosen as the detection system. The results showed that the developed method had a detection limit of 2.0 U/mL with a linear range of 0 similar to 400 U/mL. The developed method showed wider linear range than the micro-plate CLIA. The sensitivity and precision were higher than the commonly used MP-CLIA method. In addition, the method has been successfully applied to detection of CA125 in human serum with good correlation compared with the Roche method. |
| WOS记录号 | WOS:000274509200009 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21108]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li Zhiyong,Xiao Qin,Ying Xitang,et al. Micro-plate Magnetic Chemiluminescence Immunoassay of Carbohydrate Antigen 125 in Serum[J]. ACTA CHIMICA SINICA,2010,68(2):162-168. |
| APA | Li Zhiyong,Xiao Qin,Ying Xitang,Li Zhenjia,&Lin Jinming.(2010).Micro-plate Magnetic Chemiluminescence Immunoassay of Carbohydrate Antigen 125 in Serum.ACTA CHIMICA SINICA,68(2),162-168. |
| MLA | Li Zhiyong,et al."Micro-plate Magnetic Chemiluminescence Immunoassay of Carbohydrate Antigen 125 in Serum".ACTA CHIMICA SINICA 68.2(2010):162-168. |
入库方式： OAI收割
来源：生态环境研究中心
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