NMR characterization and molecular modeling of fucoidan showing the importance of oligosaccharide branching in its anticomplementary activity
文献类型：期刊论文
| 作者 | Clement, Marie-Jeanne ; Tissot, Berangere ; Chevolot, Lionel ; Adjadj, Elisabeth ; Du, Yuguo ; Curmi, Patrick A. ; Daniel, Regis |
| 刊名 | GLYCOBIOLOGY
 |
| 出版日期 | 2010-07 |
| 卷号 | 20期号:7页码:883-894 |
| 关键词 | complement system fucoidan molecular modeling protein C4 STD-NMR |
| 中文摘要 | Fucoidan is a potent inhibitor of the human complement system whose activity is mediated through interactions with certain proteins belonging to the classical pathway, particularly the protein C4. Branched fucoidan oligosaccharides displayed a higher anticomplementary activity as compared to linear structures. Nuclear magnetic resonance (NMR) characterization of the branched oligosaccharides and saturation transfer difference-NMR experiment of the interaction with the protein C4 allowed the identification of the glycan residues in close contact with the target protein. Transferred nuclear Overhauser effect spectroscopy experiment and molecular modeling of fucoidan oligosaccharides indicated that the presence of side chains reduces the flexibility of the oligosaccharide backbone, which thus adopts a conformation which is very close to the one recognized by the protein C4. Together, these results suggest that branching of fucoidan oligosaccharides, determining their conformational state, has a major impact on their anticomplementary activity. |
| WOS记录号 | WOS:000278437800008 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21112]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Clement, Marie-Jeanne,Tissot, Berangere,Chevolot, Lionel,et al. NMR characterization and molecular modeling of fucoidan showing the importance of oligosaccharide branching in its anticomplementary activity[J]. GLYCOBIOLOGY,2010,20(7):883-894. |
| APA | Clement, Marie-Jeanne.,Tissot, Berangere.,Chevolot, Lionel.,Adjadj, Elisabeth.,Du, Yuguo.,...&Daniel, Regis.(2010).NMR characterization and molecular modeling of fucoidan showing the importance of oligosaccharide branching in its anticomplementary activity.GLYCOBIOLOGY,20(7),883-894. |
| MLA | Clement, Marie-Jeanne,et al."NMR characterization and molecular modeling of fucoidan showing the importance of oligosaccharide branching in its anticomplementary activity".GLYCOBIOLOGY 20.7(2010):883-894. |
入库方式： OAI收割
来源：生态环境研究中心
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