Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N-2-dG adducts by capillary electrophoresis immunoassay
文献类型：期刊论文
| 作者 | Wang, Chao; Li, Tao; Wang, Zhixin; Feng, Feng; Wang, Hailin |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2010-04-09 |
| 卷号 | 1217期号:15页码:2254-2261 |
| 关键词 | Stereochemistry Capillary electrophoresis Immunoassays Benzo[a]pyrene DNA adducts |
| 中文摘要 | The stereospecific binding of monoclonal antibody (mAb) 8E11 to anti-benzo(a)pyrene diol epoxide (BPDE)-dG adducts in single nucleoside, long oligonucleotide, and genomic DNA were quantitatively evaluated using noncompetitive and competitive capillary electrophoresis (CE) immunoassays. Two single-stranded TMR-BPDE-90mers containing a single anti-BPDE-dG adduct with defined stereochemistry and a fluorescent label at 5'-end were used as fluorescent probes for competitive CE immunoassay. To quantitatively evaluate the binding affinity through competitive CE immunoassays, a series of equations were derived according to the binding stoichiometry. The binding of mAb 8E11 to trans-(+)-anti-BPDE-dG displays strongest affinity (K-b: 3.57 x 10(8) M-1)among all four investigated anti-BPDE-dG mononucleoside adducts, and the cis-(-)-anti-BPDE-dG displays lowest affinity (K-b: 1.14 x 10(7) M-1). The binding of monoclonal antibody (mAb) 8E11 to BPDE-dG adducts in long DNA (90mer) preferentially forms the complex with a stoichiometry of 1:1, and that mAb 8E11 displays a slightly higher affinity with trans-(+)-anti-BPDE-90mers (K-b: 6.36 +/- 0.54 x 10(8) M-1) than trans-(-)-anti-BPDE-90mers (K-b: 4.52 +/- 0.52 x 10(8) M-1). The mAb 8E11 also displays high affinity with BPDE-dG adducts in genomic DNA (K-b: 3.74 x 10(8) M-1), indicating its promising applications for sensitive immuno-detection of BPDE-DNA adducts in genomic DNA. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000276747700009 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21132]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Chao,Li, Tao,Wang, Zhixin,et al. Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N-2-dG adducts by capillary electrophoresis immunoassay[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(15):2254-2261. |
| APA | Wang, Chao,Li, Tao,Wang, Zhixin,Feng, Feng,&Wang, Hailin.(2010).Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N-2-dG adducts by capillary electrophoresis immunoassay.JOURNAL OF CHROMATOGRAPHY A,1217(15),2254-2261. |
| MLA | Wang, Chao,et al."Quantitative study of stereospecific binding of monoclonal antibody to anti-benzo(a)pyrene diol epoxide-N-2-dG adducts by capillary electrophoresis immunoassay".JOURNAL OF CHROMATOGRAPHY A 1217.15(2010):2254-2261. |
入库方式： OAI收割
来源：生态环境研究中心
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