DNA Nanostructure-Based Magnetic Beads for Potentiometric Aptasensing
文献类型：期刊论文
| 作者 | Ding, Jiawang ; Gu, Yue; Li, Fei; Zhang, Hongxia; Qin, Wei1
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| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2015-07-07 |
| 卷号 | 87期号:13页码:6465-6469 |
| 关键词 | HYBRIDIZATION CHAIN-REACTION BISPHENOL-A LABEL-FREE ELECTROCHEMICAL DETECTION MEMBRANE ELECTRODES SENSORS APTAMER COMPLEX AMPLIFICATION PROTAMINE |
| ISSN号 | 0003-2700 |
| 产权排序 | (1) Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai; Shandong, China |
| 通讯作者 | Qin, Wei |
| 英文摘要 | In this work, a simple, general, and sensitive potentiometric platform is presented, which allows potentiometric sensing to be applied to any class of molecule irrespective of the analyte charge. DNA nanostructures are self-assembled on magnetic beads via the incorporation of an aptamer into a hybridization chain reaction. The aptamer target binding event leads to the disassembly of the DNA nanostructures, which results in a dramatic change in the surface charge of the magnetic beads. Such a surface charge change can be sensitively detected by a polycation-sensitive membrane electrode using protamine as an indicator. With an endocrine disruptor bisphenol A as a model, the proposed potentiometric method shows a wide linear range from 0.1 to 100 nM with a low detection limit of 80 pM (3 sigma). The proposed sensing strategy will lay a foundation for the development of potentiometric sensors for highly sensitive and selective detection of various targets. |
| 学科主题 | Potentiometers (electric measuring instruments) |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | HYBRIDIZATION CHAIN-REACTION ; BISPHENOL-A ; LABEL-FREE ; ELECTROCHEMICAL DETECTION ; MEMBRANE ELECTRODES ; SENSORS ; APTAMER ; COMPLEX ; AMPLIFICATION ; PROTAMINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357839700008 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/8843]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Jiawang,Gu, Yue,Li, Fei,et al. DNA Nanostructure-Based Magnetic Beads for Potentiometric Aptasensing[J]. ANALYTICAL CHEMISTRY,2015,87(13):6465-6469. |
| APA | Ding, Jiawang,Gu, Yue,Li, Fei,Zhang, Hongxia,&Qin, Wei.(2015).DNA Nanostructure-Based Magnetic Beads for Potentiometric Aptasensing.ANALYTICAL CHEMISTRY,87(13),6465-6469. |
| MLA | Ding, Jiawang,et al."DNA Nanostructure-Based Magnetic Beads for Potentiometric Aptasensing".ANALYTICAL CHEMISTRY 87.13(2015):6465-6469. |
入库方式： OAI收割
来源：烟台海岸带研究所
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