Implementation of real-time two-dimensional nuclear magnetic resonance spectroscopy for on-flow high-performance liquid chromatography
文献类型：期刊论文
| 作者 | Zhou, Zhiming; Lan, Wenxian; Zhang, Weinong; Zhang, Xu; Xia, Sheng'an; Zhu, Hang; Ye, Chaohui; Liu, Maili |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2007-06-22 |
| 卷号 | 1154期号:1-2页码:464-468 |
| 关键词 | HPLC NMR HPLC-2D-NMR Hadamard amino acids urine |
| 英文摘要 | Directly coupled HPLC-NMR has become a powerful tool for separation and structural elucidation of unknown compounds. However, there are only a few reports on application of on-flow two-dimensional (2D) NMR in HPLC-NMR. Here we present an alternative method for recording realtime 2D-NMR spectrum (total correlation spectroscopy, TOCSY) on a commercial HPLC-NMR system. The method is based on well-established Hadamard matrix for 2D-NMR frequency encoding. In addition, a modified/improved solvent suppression approach is incorporated. This makes it possible to carry out the experiment with both polar and gradient eluents, the widely used chromatographic conditions. The method is exampled using a synthesized mixture of three amino acids (His, Phe and Try) and a human urine sample. The method demonstrated here may be utilized for high-throughput structural or unknown component identification and fast dynamic study in a variety of applications. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | SOLID-PHASE EXTRACTION ; LC-NMR-MS ; MASS-SPECTROMETRY ; HADAMARD SPECTROSCOPY ; STRUCTURE ELUCIDATION ; 2D NMR ; COMBINATORIAL CHEMISTRY ; DRUG DISCOVERY ; HPLC-NMR ; SPE-NMR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247419300056 |
| 公开日期 | 2015-08-18 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/4882]  |
| 专题 | 武汉物理与数学研究所_2011年以前论文发表（包括2011年） |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 2.Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Zhiming,Lan, Wenxian,Zhang, Weinong,et al. Implementation of real-time two-dimensional nuclear magnetic resonance spectroscopy for on-flow high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2007,1154(1-2):464-468. |
| APA | Zhou, Zhiming.,Lan, Wenxian.,Zhang, Weinong.,Zhang, Xu.,Xia, Sheng'an.,...&Liu, Maili.(2007).Implementation of real-time two-dimensional nuclear magnetic resonance spectroscopy for on-flow high-performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1154(1-2),464-468. |
| MLA | Zhou, Zhiming,et al."Implementation of real-time two-dimensional nuclear magnetic resonance spectroscopy for on-flow high-performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1154.1-2(2007):464-468. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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