Potentiation of ganciclovir toxicity in the herpes simplex virus thymidine kinase/ganciclovir administration system by ponicidin
文献类型：期刊论文
| 作者 | Hayashi, K; Hayashi, T; Sun, HD ; Takeda, Y
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| 刊名 | CANCER GENE THERAPY
 |
| 出版日期 | 2000 |
| 卷号 | 7期号:1页码:45-52 |
| 关键词 | herpes simplex virus type 1 thymidine kinase transfected tumor cells cytotoxicity combination therapy |
| 英文摘要 | The herpes simplex virus thymidine kinase (HSV-TK)/ganciclovir (CCV) administration system is commonly used in gene therapy trials. We have evaluated the effect of ponicidin, a diterpenoid isolated from a plant, Rabdosia ternifolia, on the cell-killing activity of the anti-herpes drugs acyclovir (ACV) and CCV. Ponicidin preferentially activated HSV-1-specific TK but not cellular kinases. In HSV-infected cells, ponicidin significantly accumulated the phosphorylated metabolites of CCV and suppressed the extracellular release of CCV. These data suggested that the cytotoxicities of ACV and CCV in HSV-TK-expressing cells might be potentiated by ponicidin. After transfected with the HSV-1 TK gene, COS-1 and several human cancer cells became highly sensitive to the cytotoxic properties of the nucleoside analogs. When ponicidin at the concentration without antiviral activities (0.2 mu g/mL) was combined with ACV or GCV, the cytotoxic levels in HSV-TK-expressing cells were enhanced by 3- to 87-fold and 5- to 52-fold, respectively, compared with the nucleoside alone. When the stability of the bioactivity of ponicidin in the blood of mice was evaluated, the substance showed relatively long-lasting effects on the potentiation of the anti-herpetic and cytotoxic activities of GCV after intravenous administration. These data suggest that the combined use of ponicidin with CCV will be effective for cancer gene therapy, because high cytotoxicity in viral TK-expressing cells should yield more rapid and enhanced tumor elimination. |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Oncology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Oncology ; Genetics & Heredity ; Research & Experimental Medicine |
| 关键词[WOS] | MEDIATED GENE-TRANSFER ; INDUCED DNA-POLYMERASE ; BRAIN-TUMORS ; KINASE ; ACYCLOVIR ; CELLS ; 9-(1,3-DIHYDROXY-2-PROPOXYMETHYL)GUANINE ; 9-(2-HYDROXYETHOXYMETHYL)GUANINE ; REPLICATION ; PHOSPHORYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000085401500006 |
| 公开日期 | 2015-08-18 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/23965]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Toyama Med & Pharmaceut Univ, Dept Virol, Toyama 9300194, Japan 2.Toyama Med & Pharmaceut Univ, Fac Pharmaceut Sci, Toyama 9300194, Japan 3.Acad Sinica, Kunming Inst Bot, Kunming 650107, Yunnan, Peoples R China 4.Univ Tokushima, Fac Integrated Arts & Sci, Tokushima 770, Japan |
| 推荐引用方式 GB/T 7714 | Hayashi, K,Hayashi, T,Sun, HD,et al. Potentiation of ganciclovir toxicity in the herpes simplex virus thymidine kinase/ganciclovir administration system by ponicidin[J]. CANCER GENE THERAPY,2000,7(1):45-52. |
| APA | Hayashi, K,Hayashi, T,Sun, HD,&Takeda, Y.(2000).Potentiation of ganciclovir toxicity in the herpes simplex virus thymidine kinase/ganciclovir administration system by ponicidin.CANCER GENE THERAPY,7(1),45-52. |
| MLA | Hayashi, K,et al."Potentiation of ganciclovir toxicity in the herpes simplex virus thymidine kinase/ganciclovir administration system by ponicidin".CANCER GENE THERAPY 7.1(2000):45-52. |
入库方式： OAI收割
来源：昆明植物研究所
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