Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR
文献类型：期刊论文
| 作者 | Xue, Chun-Ying2 ; Li, De-Zhu2; Wang, Qing-Zhong1
|
| 刊名 | PLANTA MEDICA
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 74期号:15页码:1842-1845 |
| 关键词 | DNA Gentiana macrophylla Gentianaceae real-time PCR species identification |
| 英文摘要 | Gentiana macrophylla Pall. is a commonly used antirheumatic herb. There are four species of Gentiona recorded as herbal drugs in the Chinese Pharmacopoeia. The other species are often marketed as G. macrophylla, and thus the therapeutic effects of G. macrophylla are not achieved. A novel one-step methodology based on real-time polymerase chain reaction (PCR) technology has been developed for the identification of G. macrophylla. This relative quantification methodology does not require a known amount of standard, allowing the analysis of many more samples together. The utilization of real-time PCR does not require sample handling, preventing contamination and resulting in much faster and higher throughput results. |
| 类目[WOS] | Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Plant Sciences ; Pharmacology & Pharmacy |
| 关键词[WOS] | RIBOSOMAL DNA ; AUTHENTICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262107800017 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/23867]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Yunnan Univ, Sch Chem Sci & Technol, Kunming 610091, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Lab Plant Biodivers & Biogeog, Kunming, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Chun-Ying,Li, De-Zhu,Wang, Qing-Zhong. Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR[J]. PLANTA MEDICA,2008,74(15):1842-1845. |
| APA | Xue, Chun-Ying,Li, De-Zhu,&Wang, Qing-Zhong.(2008).Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR.PLANTA MEDICA,74(15),1842-1845. |
| MLA | Xue, Chun-Ying,et al."Identification and Quantification of the Traditional Chinese Medicinal Plant Gentiana macrophylla using Taqman Real-Time PCR".PLANTA MEDICA 74.15(2008):1842-1845. |
入库方式： OAI收割
来源：昆明植物研究所
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