Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds
文献类型：期刊论文
| 作者 | Roach, Thomas1,2; Beckett, Richard P.3; Minibayeva, Farida V.4; Colville, Louise1; Whitaker, Claire3; Chen, Hongying1,5; Bailly, Christophe6; Kranner, Ilse1 |
| 刊名 | PLANT CELL AND ENVIRONMENT
 |
| 出版日期 | 2010 |
| 卷号 | 33期号:1页码:59-75 |
| 关键词 | Castanea sativa germination glutathione hydrogen peroxide peroxidase phenolic acid reactive oxygen species seed superoxide |
| 英文摘要 | Reactive oxygen species (ROS) are implicated in seed death following dehydration in desiccation-intolerant 'recalcitrant' seeds. However, it is unknown if and how ROS are produced in the apoplast and if they play a role in stress signalling during desiccation. We studied intracellular damage and extracellular superoxide (O-2 center dot-) production upon desiccation in Castanea sativa seeds, mechanisms of O-2 center dot- production and the effect of exogenously supplied ROS. A transient increase in extracellular O-2 center dot- production by the embryonic axes preceded significant desiccation-induced viability loss. Thereafter, progressively more oxidizing intracellular conditions, as indicated by a significant shift in glutathione half-cell reduction potential, accompanied cell and axis death, coinciding with the disruption of nuclear membranes. Most hydrogen peroxide (H2O2)-dependent O-2 center dot- production was found in a cell wall fraction that contained extracellular peroxidases (ECPOX) with molecular masses of similar to 50 kDa. Cinnamic acid was identified as a potential reductant required for ECPOX-mediated O-2 center dot- production. H2O2, applied exogenously to mimic the transient ROS burst at the onset of desiccation, counteracted viability loss of sub-lethally desiccation-stressed seeds and of excised embryonic axes grown in tissue culture. Hence, extracellular ROS produced by embryonic axes appear to be important signalling components involved in wound response, regeneration and growth. |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | PROGRAMMED CELL-DEATH ; FREE-RADICAL PROCESSES ; INDICA JUSS,A. SEEDS ; HYDROGEN-PEROXIDE ; OXIDATIVE STRESS ; PLASMA-MEMBRANE ; NADPH OXIDASE ; HORSERADISH-PEROXIDASE ; HYDROXYL RADICALS ; PLANT PEROXIDASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272661000006 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/23910]  |
| 专题 | 昆明植物研究所_中国西南野生生物种质资源库 |
| 作者单位 | 1.Royal Bot Gardens Kew, Seed Conservat Dept, Ardingly RH17 6TN, W Sussex, England 2.UCL, Res Dept Genet Evolut & Environm, London WC1E 6BT, England 3.Univ KwaZulu Natal, Sch Biol & Conservat Sci, ZA-3209 Pietermaritzburg, Scottsville, South Africa 4.Russian Acad Sci, Kazan Inst Biochem & Biophys, Kazan 420111, Russia 5.Chinese Acad Sci, Kunming Inst Bot, Kunming 661100, Peoples R China 6.Univ Paris 06, EA2388, F-75005 Paris, France |
| 推荐引用方式 GB/T 7714 | Roach, Thomas,Beckett, Richard P.,Minibayeva, Farida V.,et al. Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds[J]. PLANT CELL AND ENVIRONMENT,2010,33(1):59-75. |
| APA | Roach, Thomas.,Beckett, Richard P..,Minibayeva, Farida V..,Colville, Louise.,Whitaker, Claire.,...&Kranner, Ilse.(2010).Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds.PLANT CELL AND ENVIRONMENT,33(1),59-75. |
| MLA | Roach, Thomas,et al."Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds".PLANT CELL AND ENVIRONMENT 33.1(2010):59-75. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。