Time-resolved two-photon excitation fluorescence spectroscopy
文献类型：期刊论文
| 作者 | Liu Li-Xin; Qu Jun-Le; Lin Zi-Yang; Chen Dan-Ni; Xu Gai-Xia; Hu Tao; Guo Bao-Ping; Niu Han-Ben |
| 刊名 | acta physica sinica
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 55期号:12页码:6281-6286 |
| 关键词 | fluorescence lifetime fluorescence spectrum two-photon excitation high repetition rate streak camera |
| 英文摘要 | a novel time-resolved two-photon excitation fluorescence spectroscopic system based on a high repetition rate streak camera and capable of simultaneous measurement of fluorescence spectrum and lifetime of the samples is presented. the system has high temporal (6.5-200 ps) and spectral ( 1-3 nm) resolutions and permits rapid data acquisition and reliable and reproducible lifetime and spectrum determinations. the system is tested with standard fluorescent dyes (rhodamine 6g, coumarin 314 and their mixtures) and the lifetimes and spectra obtained agree well with those reported in the literatures. experimental results indicate that our system provides a unique contrast mechanism for discriminating multi-fluorophore or multi-component of biological tissues through spectrally and temporally resolved information and may find extensive applications in multispectral fluorescence lifetime imaging and fluorescence resonance energy transfer imaging. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | physics, multidisciplinary |
| 研究领域[WOS] | physics |
| 关键词[WOS] | imaging microscopy ; streak camera ; quantum dots ; lifetime ; diagnostics ; cells |
| 收录类别 | SCI ; EI |
| 语种 | 中文 |
| WOS记录号 | WOS:000243900800015 |
| 公开日期 | 2015-08-25 |
| 源URL | [http://ir.opt.ac.cn/handle/181661/25178]  |
| 专题 | 西安光学精密机械研究所_研究生部 |
| 作者单位 | 1.Shenzen Univ, Inst Optoelect, Shenzhen 518060, Peoples R China 2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, Xian 710068, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Li-Xin,Qu Jun-Le,Lin Zi-Yang,et al. Time-resolved two-photon excitation fluorescence spectroscopy[J]. acta physica sinica,2006,55(12):6281-6286. |
| APA | Liu Li-Xin.,Qu Jun-Le.,Lin Zi-Yang.,Chen Dan-Ni.,Xu Gai-Xia.,...&Niu Han-Ben.(2006).Time-resolved two-photon excitation fluorescence spectroscopy.acta physica sinica,55(12),6281-6286. |
| MLA | Liu Li-Xin,et al."Time-resolved two-photon excitation fluorescence spectroscopy".acta physica sinica 55.12(2006):6281-6286. |
入库方式： OAI收割
来源：西安光学精密机械研究所
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