The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish
文献类型：期刊论文
| 作者 | Huang, Heng; Jin, Ting; Wang, Lin; Wang, Fulong; Zhang, Rui; Pan, Yi（潘怡）; Wang, ZhenZhen（王甄真）; Chen, Yan（陈雁）
|
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2013 |
| 卷号 | 288期号:4页码:2355-2364 |
| 英文摘要 | The molecular basis of the lymphatic development remains largely unknown. Using zebrafish as a model, we discovered a novel role for the Ras guanine-releasing protein 1 (RasGRP1), a protein involved in Ras activation in lymphangiogenesis. Secondary lymphatic sprouts from the posterior cardinal vein give rise to thoracic duct which is the first lymphatic vessel in zebrafish. Knockdown of rasgrp1 by injecting morpholino in zebrafish embryos impaired formation of thoracic duct accompanied by pericardial and truck edema, whereas blood vessel development of the embryos was largely unaffected. In rasgrp1-knockdown embryos, the number of sprouts producing the string of parachordal lymphangioblast cells was reduced. Meanwhile the total number of the secondary sprouts was not changed. As a result, the number of venous intersegmental vessels was increased, whereas the number of lymphatic vessel was reduced at a later stage. The lymphatic developmental defects caused by rasgrp1 knockdown could be rescued by ectopic expression of a constitutively active HRas. Further analysis revealed that RasGRP1 knockdown could synergize with flt4/vegfr3 knockdown to induce defects in lymphangiogenesis. Taken together, this finding demonstrates a critical role for RasGRP1 in lymphatic development in zebrafish. |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ENDOTHELIAL-GROWTH-FACTOR ; VASCULAR DEVELOPMENT ; FACTOR-C ; IN-VIVO ; SYSTEM ; LYMPHANGIOGENESIS ; DOWNSTREAM ; ACTIVATION ; MECHANISMS ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000314211500024 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/63]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所_信号转导与营养相关疾病研究组 |
| 推荐引用方式 GB/T 7714 | Huang, Heng,Jin, Ting,Wang, Lin,et al. The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(4):2355-2364. |
| APA | Huang, Heng.,Jin, Ting.,Wang, Lin.,Wang, Fulong.,Zhang, Rui.,...&Chen, Yan.(2013).The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish.JOURNAL OF BIOLOGICAL CHEMISTRY,288(4),2355-2364. |
| MLA | Huang, Heng,et al."The RAS Guanyl Nucleotide-releasing Protein RasGRP1 Is Involved in Lymphatic Development in Zebrafish".JOURNAL OF BIOLOGICAL CHEMISTRY 288.4(2013):2355-2364. |
入库方式： OAI收割
来源：上海营养与健康研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。